土壤中酚类化合物降解菌筛选及应用

孟宪荣; 许伟; 魏蓝; 贾超; 蒋村

doi:10.3969/j.issn.1674-991X.2018.04.059

土壤中酚类化合物降解菌筛选及应用

doi: 10.3969/j.issn.1674-991X.2018.04.059

孟宪荣^1,,
许伟¹,
魏蓝²,
贾超²,
蒋村²

^1. 苏州市环境科学研究所,江苏苏州 215007
^2. 苏州科技大学环境工程学院,江苏苏州 215009

详细信息

作者简介:
孟宪荣(1989—),女,工程师,博士,主要从事土壤污染防治与修复技术研究

中图分类号: X53
计量
- 文章访问数: 659
- HTML全文浏览量: 52
- PDF下载量: 414
- 被引次数: 0
出版历程
- 收稿日期: 2018-01-22
- 刊出日期: 2018-07-20

Screening and primary application of phenols degradation strains in soil

^1. Suzhou Environmental Science Research Institute, Suzhou 215007, China
^2. School of Environmental Engineering, University of Science and Technology of Suzhou, Suzhou 215009, China

摘要

摘要: 以苏州某化工场地污染土壤为菌源,以目标污染物为唯一碳源,通过连续培养、分离纯化筛选出高效酚类化合物降解菌。利用16S rDNA基因测序、构建基因树等分子生物学技术手段确定菌株种属,并研究了菌株对酚类化合物耐受性和降解效果。结果表明:以4-甲基苯酚(4-MP)和4-氯-3-甲基苯酚(4-C-3-MP)为唯一碳源筛选出了2株菌,分别为嗜麦芽窄食单胞菌(NM)和香茅醇假单胞菌(NCM)。菌株NM对4-MP耐受性良好,4-MP浓度为400 mg/L时仍能生长繁殖;菌株NCM对4-C-3-MP耐受性较差,4-C-3-MP浓度≥200 mg/L时,菌株NCM受到完全抑制,停止生长。菌株NM不能降解土壤中苯酚,对4-MP和4-C-3-MP去除效果良好,第20天时去除率分别达到43%和22%;菌株NCM对土壤中苯酚、4-MP和4-C-3-MP均有降解效果,第20天时苯酚、4-MP和4-C-3-MP去除率分别为20%、26%和28%,较第15天时去除率分别提高了18、3和6个百分点。
- 土壤 /
- 微生物修复 /
- 酚类化合物 /
- 高效降解菌
Abstract: With the phenols contaminated soil from a site in Suzhou City as the source of bacterium, and the target contaminant as the only carbon source, the phenols degrading bacteria were screened out by the way of continuous culture, separation and purification. By applying the molecular biological techniques of 16S rDNA gene sequencing and gene trees, the bacterium species were determined. The tolerance and degradation capacity of the bacterium were also investigated. The results showed that two strains of bacteria were screened out, one was Stenotrophomonas maltophilia strain with p-cresol(4-MP) as the only carbon source, and the other was Pseudomonas citronellolis strain with 4-chloro-3-methylphenol(4-C-3-MP) as the only carbon source, which are named bacteria NM and bacteria NCM, respectively. Bacteria NM was well tolerated to 4-MP, which could still propagate when the concentration of 4-MP reached 400 mg/L. However, bacteria of NCM could not tolerate high concentration 4-C-3-MP. When concentration of 4-C-3-MP was higher than 200 mg/L, bacteria NCM was completely suppressed. Bacteria NM cannot degrade phenol in soil, but has high removal efficiency on 4-MP and 4-C-3-MP, and after 20 days, the removal efficiency of 4-MP and 4-C-3-MP were about 43% and 22% respectively. Bacteria NCM can well degrade the three phenols. The removal efficiency of phenol, 4-MP and 4-C-3-MP by bacteria NCM were 20%, 26% and 28% at the 20 ^th day, with an increase by 18, 3 and 6 percentage points respectively compared with that at the 15th day.
- soil /
- microbial remediation /
- phenols /
- high-effective degradation strain

HTML全文

参考文献(28)

[1]	范聪, 肖炜, 张仕颖 . 微生物修复污染土壤的应用研究进展[J]. 贵州农业科学, 2017,45(8):53-58. FAN C, XIAO W, ZHANG S Y . Advances in application of microorganisms in contaminated soil[J]. Guizhou Agricultural Sciences, 2017,45(8):53-58.
[2]	孙玉军, 王效科, 王如松 . 五指山保护区生态环境质量评价研究[J]. 生态学报, 1999,19(3):365-370. doi: 10.3321/j.issn:1000-0933.1999.03.013
[3]	刘张, 周成 . 污染土壤微生物修复技术[J]. 生物技术世界, 2013(1), 18-20.
[4]	黄廷林, 唐智新, 徐金兰 , 等. 黄土地区石油污染土壤生物修复室内模拟试验研[J]. 农业环境科学学报, 2008,27(6):2206-2210. HUANG T L, TANG Z X, XU J L , et al. Laboratory study on bioremediation of petroleum-contaminated soil in loess region of China[J]. Journal of Agro-Environment Science, 2008,27(6):2206-2210.
[5]	乔俊, 陈威, 张承东 . 添加不同营养助剂对石油污染土壤生物修复的影响[J]. 环境化学, 2010,29(1):6-11. QIAO J, CHEN W, ZHANG C D . Bioremdiation of petroleum on taminated soil by verious nutrient amendments[J]. Evironmental Chemistry, 2010,29(1):6-11.
[6]	SARKAR D, FERGUSON M, DATTA R , et al. Bioremediation of petroleum hydrocarbons in contaminated soils:comparison of biosolids addition,carbon supplementation,and monitored natural attenuation[J]. Environmental Pollution, 2005,136(1):187-195. doi: 10.1016/j.envpol.2004.09.025 pmid: 15809120
[7]	AYOTAMUNO M J, KOGBARA R B, OGAJI S O T ,et al. Bioremediation of a crude-oil polluted agricultural soil at Port Harcourt,Nigeria[J]. Applied Energy, 2006,83:1249-1257. doi: 10.1016/j.apenergy.2006.01.003
[8]	牛明芬, 韩晓日, 郭书海 . 生物表面活性剂在石油污染土壤生物预制床修复中的应用研究[J]. 土壤通报, 2005,36(5):712-715. NIU M F, HAN X R, GUO S H . Application of biosurfactant in the biodegradation of petroleum contaminated soil with bio-prepared bed[J]. Chinese Journal of Soil Science, 2005,36(5):712-715.
[9]	张秀霞, 滕芝, 吴佳东 . 激活剂对石油污染土壤修复的强化作用及修复条件的优化[J]. 石油学报, 2013,29(2):353-358. doi: 10.3969/j.issn.1001-8719.2013.02.027 ZHANG X X, TENG Z, WU J D . Strengthening bioremedation of oil contaminated soil by activation agent and optimization of bioremediation conditions[J]. Acta Petrolei Sinica, 2013,29(2):353-358. doi: 10.3969/j.issn.1001-8719.2013.02.027
[10]	李春荣 . 石油污染土壤的生态效应及生物修复研究[D]. 西安:长安大学, 2009.
[11]	RAHMAN K S M, BANAT I M, THAHIRA J , et al. Bioremediation of gasoline contaminated soil by a bacterial consortium amended with poultry litter,coir pith,and rhamnolipid biosurfactant[J]. Bioresource Technology, 2002,81(1):25-32. doi: 10.1016/S0960-8524(01)00105-5 pmid: 11710344
[12]	魏小芳, 张忠智, 郭绍辉 , 等. 外源微生物强化修复石油污染土壤的研究[J]. 石油化工高等学校学报, 2007,20(2):1-4. doi: 10.3969/j.issn.1006-396X.2007.02.001 WEI X F, ZHANG Z Z, GUO S H , et al. Ex site-bio remediation of petroleum-contaminated soil with exogenous microbe[J]. Journal of Petrochemical Universities, 2007,20(2):1-4. doi: 10.3969/j.issn.1006-396X.2007.02.001
[13]	张秀霞, 滕芝, 吴佳东 . 生物强化修复石油污染土壤[J]. 环境工程学报, 2013,7(4):1573-1577. ZHANG X X, TENG Z, WU J D . Bioaugmentation remediation of petroleum contaminated soil[J]. Chinese Journal of Environmental Engineering, 2013,7(4):1573-1577.
[14]	屠明明, 王秋玉 . 石油污染土壤的生物刺激和生物强化修复[J]. 中国生物工程杂志, 2009,29(8):129-134. TU M M, WANG Q Y . Biostmiulation and bioaugmentation repair of oil contaminated soil[J]. China Biotechnology, 2009,29(8):129-134.
[15]	宋雪英, 宋玉芳, 孙铁珩 , 等. 矿物油污染土壤中芳烃组分的生物降解与微生物生长动态[J]. 环境科学, 2004,25(3):115-119. doi: 10.3321/j.issn:0250-3301.2004.03.025 SONG X Y, SONG Y F, SUN T H , et al. Biodegradation of aromatic hydrocarbons and dynamics of microbe growth in soils contaminated with mineral oil[J]. Environmental Science, 2004,25(3):115-119. doi: 10.3321/j.issn:0250-3301.2004.03.025
[16]	焦海华, 潘建刚, 徐圣君 , 等. 原位生物修复提高多环芳烃污染土壤农用安全性[J]. 环境科学, 2015,36(8):3038-3044. doi: 10.13227/j.hjkx.2015.08.042 JIAO H H, PAN J G, XU S J , et al. Improving agricultural safety of soils contaminated with polycyclic aromatic hydrocarbons by in situ bioremediation[J]. Environmental Science, 2015,36(8):3038-3044. doi: 10.13227/j.hjkx.2015.08.042
[17]	LU L, YAZDI H, JIN S , et al. Enhanced bioremediation of hydrocarbon-contaminated soil using pilot-scale bioelectrochemical systems[J]. Journal of Hazardous Materials, 2014,274:8-15. doi: 10.1016/j.jhazmat.2014.03.060 pmid: 24762696
[18]	陈洁霞, 韦恩泽, 鲜啟鸣 . 长江沿岸某化工园区土壤、底泥中酚类化合物的污染现状[J]. 色谱, 2014,32(8):843-848. doi: 10.3724/SP.J.1123.2014.03041 CHEN J X, WEI E Z, XIAN Q M . Pollution status of phenolic compounds in the soil and sediment from a chemical industrial park along the Yangtze River[J]. Chinese Journal of Chromatography, 2014,32(8):843-848. doi: 10.3724/SP.J.1123.2014.03041
[19]	LI L H, ZHANG P Y, ZHU W P . Comparison of O₃-BAC,UV/O₃-BAC and TiO₂/UV/O₃-BAC processes for removing organic pollutants in secondary effluents[J]. Journal of Photochemistry and Photobiology A:Chemistry, 2005,171:145-151. doi: 10.1016/j.jphotochem.2004.09.016
[20]	李日强, 王翠红, 辛晓芸 , 等. 焦化废水处理中酚、氰降解细菌的分离选育[J]. 重庆环境科学, 2002,24(3):23-24. doi: 10.3969/j.issn.1674-2842.2002.03.009 LI R Q, WANG C H, XIN X Y , et al. Isolation and screening of degrading cyanide and phenol bacteria in the coke wastewater treatment[J]. Environment Science of Chongqing, 2002,24(3):23-24. doi: 10.3969/j.issn.1674-2842.2002.03.009
[21]	陈明, 张维, 徐玉泉 , 等. 醋酸钙不动杆菌PHEA-2对苯酚的降解特性研究[J]. 中国环境科学, 2001,21(3):226-229. doi: 10.3321/j.issn:1000-6923.2001.03.008 CHEN M, ZHANG W, XU Y Q , et al. Study on characteristics of Acinetobater calcoaceticus PHEA-2 for phenol degradation[J]. China Environmental Science, 2001,21(3):226-229. doi: 10.3321/j.issn:1000-6923.2001.03.008
[22]	HOLLENDER J, HOPP J, DOTT W . Degradation of 4-chlorophenol via the meta cleavage pathway by Comamonas testosteroni JH5[J]. Applied and Environmental Microbiology, 1997,63(11):4567-4572.
[23]	COLE J R, CASCARELLI A L, MOHN W W , et al. Isolation and characterization of a novel bacterium growing via reductive dehalogenation of 2-chlorophenol[J]. Applied and Environmental Microbiology, 1994,60(10):3536-3542. doi: 10.1099/0022-1317-22-3-455 pmid: 201851
[24]	布坎南, 吉布斯 . 伯杰氏细菌鉴定手册[M].8版.颜子颖,王海林,译. 北京: 科学出版社, 1999.
[25]	东秀珠, 蔡妙英 . 常见细菌系统鉴定手册[M]. 北京: 科学出版社, 2001.
[26]	张纪忠 . 微生物分类学[M]. 上海: 复旦大学出版社, 1990.
[27]	刘蛟 . 菌群中关键菌株降解酚类废水、本征动力学及环境代谢组学解析[D]. 天津:天津大学, 2012.
[28]	闻欣, 薛光璞, 张群 , 等. 酚类化合物在水环境中的生物降解研究[J]. 黑龙江环境通报, 2010,34(3):63-66. WEN X, XUE G P, ZHANG Q , et al. Study on biological degradation of phenolic compounds in water[J]. Heilongjiang Environmental Journal, 2010,34(3):63-66.