Abstract: Evaluation and control of toxic pollutants in industrial wastewater is an import issue in the filed of water environmental pollution control. Only measuring quantitative integrated indexes such as COD_Cr，BOD₅ is not sufficient for evaluating the safety of wastewater. Industrial wastewater water quality indexes including routine water quality indexes, bio-toxicity indexes and chemical characteristic indexes were proposed. Bio-toxicity tests and their application in industrial wastewater evaluation were discussed. The necessity and importance to carry out wastewater safety management using integrated bio-toxicity method was pointed out. It is suggested that the application of Daphnia toxicity tests for industrial wastewater safety evaluation should be the top choice in China. In addition, the progress of wastewater bio-toxicity control criteria at home and abroad was summarized, and it was pointed out that the bio-toxicity control index and toxicity discharge criteria for industrial wastewater which was suitable for China should be established according to water environmental creature type, toxic characteristic of industrial wastewater pollutants and control technology level.