滇池藻胆蛋白时空分布特征及其影响因素分析

刘平; 魏薇; 杨俊晖; 李建文; 左李美; 孟睿; 段平洲; 焦立新

doi:10.12153/j.issn.1674-991X.20230146

滇池藻胆蛋白时空分布特征及其影响因素分析

doi: 10.12153/j.issn.1674-991X.20230146

刘平^{1, 2,},
魏薇²,
杨俊晖²,
李建文²,
左李美²,
孟睿¹,
段平洲^1, ,,
焦立新¹

1.
环境基准与风险评估国家重点实验室，中国环境科学研究院
2.
昆明市城市排水监测站

基金项目: 国家重点研发计划项目(2017YFA0605202，2021YFC3201003）；国家科技基础性工作专项重点项目(2015FY110900)；中央级公益性科研院所基本科研业务费专项（YSKY2023-10）

详细信息

作者简介:
刘平(1990—)，男，工程师，主要从事滇池流域水环境监测及分析研究，1158159563@qq.com

通讯作者:
段平洲(1990—)，男，副研究员，博士，主要从事水环境化学和污染控制技术研究，duanpz@craes.org.cn

中图分类号: X524；X705
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- 被引次数: 0
出版历程
- 收稿日期: 2023-02-23
- 网络出版日期: 2023-11-24

Spatial and temporal distribution characteristics of phycobiliprotein in Dianchi Lake and analysis of its influencing factors

1.
State Key Laboratory of Environment Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences
2.
Kunming Urban Drainage Monitoring Station

摘要

摘要:
藻胆蛋白具有重要的经济价值，可以从藻体中提取、加工，从而实现藻泥废物的减量化与资源化。为厘清滇池水体藻胆蛋白的时空分布特征及其影响因素，于2022年不同季节进行了4次采样，在建立藻胆蛋白检测方法的基础上，分析了滇池草海、外海北部、外海中部和外海南部4个区域的藻胆蛋白时空变化特征，并基于ArcGIS建立了藻胆蛋白浓度时间和空间序列栅格数据图。结果表明：1）滇池藻胆蛋白浓度在时间上呈现夏季>秋季>春季>冬季的分布特征，在空间上呈现外海北部>外海中部>外海南部>草海的规律，且以藻蓝蛋白为主要成分，藻红蛋白的浓度较低。2）通过对各影响因子的统计分析发现，藻蓝蛋白浓度与TP浓度和BOD₅存在显著的线性相关性（P<0.01），藻红蛋白浓度与营养盐的相关性较低，藻胆蛋白浓度与pH、DO浓度和透明度的线性关系显著（P<0.05）。3）对藻胆蛋白的影响因子进行分湖区讨论，结果表明氮、磷是藻胆蛋白分布的主要驱动因子，并可能受到水力条件和藻类种群类别的影响。4）草海虽然具有最高的TN、TP平均浓度，但其氮磷比（N/P）过高，蓝藻群落被绿藻取代，导致其藻胆蛋白浓度最低；外海北部具有较长的水力停留时间和较低的N/P，造成蓝藻种群占比高，藻胆蛋白的浓度最高；滇池水体春冬季的N/P显著高于夏秋季，这也是夏秋季藻胆蛋白浓度高于春冬季的主要原因之一。
- 藻胆蛋白 /
- 滇池 /
- 时空分布 /
- 环境因素 /
- 氮磷
Abstract:
Phycobiliprotein has important economic value. It can be extracted and processed from algae to realize the reduction and recycling of algae sludge waste. Aiming to clarify the spatial and temporal distribution characteristics of phycobiliprotein in Dianchi Lake and its influencing factors, four samples were collected by seasons in 2022. Upon establishing the detection method of phycobiliprotein, the spatial and temporal variation characteristics of phycobiliprotein in four areas of Dianchi Lake, namely Caohai, northern Waihai, central Waihai and southern Waihai were analyzed. Based on ArcGIS, the time and spatial sequence grid data chart of phycobiliprotein concentration in Dianchi Lake was established. The results showed that the phycobiliprotein concentrations in Dianchi Lake presented the distribution characteristics of summer>autumn>spring>winter in time, the rule of northern Waihai>central Waihai>southern Waihai>Caohai in space, and the concentration of phycobiliprotein was low with phycocyanin as the main component. Through the statistical analysis of various influencing factors, it was found that there was a strong linear correlation between phycocyanin concentration and TP and BOD₅ (P<0.01). The correlation between phycoerythrin and nutrients was low. The increase of phycobiliprotein in turn would affect pH, DO and transparency of the water body (P<0.05). The influencing factors of phycobiliprotein were discussed in different areas, and it was concluded that nitrogen and phosphorus were the main driving factors of phycobiliprotein distribution, which might be affected by hydraulic conditions and algae population types. Although Caohai had the highest average concentration of TN and TP, its N/P value was too high, and the blue-green algae community was replaced by green algae. At the same time, its phycobiliprotein concentrationd was the lowest. While the northern Waihai had long hydraulic retention time and low N/P value, resulting in a higher proportion of blue-green algae population and the highest concentration of phycobiliprotein. N/P of Dianchi Lake water body in spring and winter was significantly higher than that in summer and autumn, which was one of the main reasons for the higher concentration of phycobiliprotein in summer and autumn.
- phycobiliprotein /
- Dianchi Lake /
- spatiotemporal distribution /
- environmental factors /
- nitrogen and phosphorus

HTML全文

图 1 滇池流域水系和采样点分布

Figure 1. Water system of Dianchi Lake Basin and distribution of sampling points

下载: 全尺寸图片幻灯片

图 2 藻胆蛋白的吸光波谱和标准曲线

注：C为藻胆蛋白浓度；A为吸光度。

Figure 2. Absorption spectrum and standard curve of phycobiliprotein

下载: 全尺寸图片幻灯片

图 3 滇池藻红蛋白浓度时空分布

Figure 3. Spatial and temporal distribution of phycoerythrin concentration in Dianchi Lake

下载: 全尺寸图片幻灯片

图 4 滇池藻蓝蛋白浓度时空分布

Figure 4. Spatial and temporal distribution of phycocyanin concentration in Dianchi Lake

下载: 全尺寸图片幻灯片

图 5 滇池藻类组分与环境因子相关性分析

Figure 5. Correlation analysis of algae components and environmental factors in Dianchi Lake

下载: 全尺寸图片幻灯片

图 6 2022年滇池水体TN、TP浓度和N/P月度变化

Figure 6. Monthly variation of TN, TP concentrations and N/P in Dianchi Lake in 2022

下载: 全尺寸图片幻灯片

表 1 滇池不同湖区水体TN、TP浓度与藻胆蛋白浓度皮尔逊相关系数

Table 1. Pearson correlation coefficient between TN, TP and phycobiliprotein concentrations in different lakes of Dianchi Lake

湖区	指标	TP	TN	N/P	藻红蛋白	藻蓝蛋白
草海	TP	1	0.079	−0.613^**	0.166	0.373^*
	TN		1	0.357^*	−0.115	−0.152
	N/P			1	−0.169	−0.373^*
	藻红蛋白				1	0.371
	藻蓝蛋白					1
外海北部	TP	1	0.436^*	0.671^**	0.382^*	0.604^**
	TN		1	0.442^*	−0.321^*	−0.396^*
	N/P			1	−0.167	−0.489^*
	藻红蛋白				1	0.424^*
	藻蓝蛋白					1
外海中部	TP	1	−0.664^**	−0.713^**	0.493^*	0.637^**
	TN		1	0.397^*	−0.299	0.404^*
	N/P			1	−0.198	−0.449^*
	藻红蛋白				1	0.392^*
	藻蓝蛋白					1
外海南部	TP	1	−0.487^*	−0.498^**	0.279	0.411^*
	TN		1	0.266	−0.213	−0.374
	N/P			1	−0.179	−0.406^*
	藻红蛋白				1	0.383^*
	藻蓝蛋白					1
注：表示P<0.05，*表示P<0.01。

下载: 导出CSV

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