FeS2 enhanced microbial fuel cell anode denitrification and electricity generation characteristics
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摘要:
针对不同碳氮比（C/N）的含氮废水，将FeS2引入微生物燃料电池（MFC）阳极构建FeS2强化的微生物燃料电池（Pyr-MFC）体系，以不加FeS2的空白对照组（C-MFC）为对照，探究其对体系脱氮与产电的影响；采用高通量测序、X射线光电子能谱和扫描电子显微镜探究该体系中微生物丰度、硫和铁元素变化规律，解析FeS2强化体系低C/N下的脱氮机理。结果表明：1）Pyr-MFC的反硝化脱氮效率和产电功率密度均高于C-MFC，硝态氮去除率提高近15.7%，最高电压提高量可达0.274 V。2）C/N分别为4、3、2和1时Pyr-MFC对NO3-N的去除率为100%、97.8%、58.4%和49.7%，均高于C-MFC，表明FeS2有效降低体系对碳源的依赖。3）微生物群落检测结果表明，FeS2将产电微生物（Thauera、Thiobacillus和Geobacter）的物种丰度提高9.43%。4）物质转移分析结果表明，S−为反硝化过程提供电子，Fe2+作为电子穿梭体强化了电子传递，提高了体系的产电性能。
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关键词:
- 微生物燃料电池(MFC) /
- FeS2 /
- 强化 /
- 反硝化脱氮 /
- 产电
Abstract:In recent years, the research on enhanced microbial fuel cell (MFC) treatment of nitrogen-containing wastewater has attracted extensive attention at home and abroad. FeS2 was introduced into MFC anode to construct a FeS2-enhanced microbial fuel cell (Pyr-MFC) system for wastewater with different carbon to nitrogen ratios (C/N). The blank control group without FeS2 (C-MFC) was used as a control to explore its effects on nitrogen removal and electro-generation. High throughput sequencing, X-ray photoelectron spectroscopy (XPS) and scanning electron microscopy (SEM) were used to investigate the changes of microbial abundance, sulfur and iron elements in the system, and the mechanism of nitrogen removal under low C/N in FeS2-enhanced system was obtained. The results showed: 1) The denitrification efficiency and power generation density of Pyr-MFC were higher than those of the control group, the nitrate nitrogen removal rate was increased by 15.7%, and the maximum voltage increase was as high as 0.274 V. 2) NO3 −-N removal rates of Pyr-MFC at different C/N ratios (4, 3, 2 and 1) were 100%, 97.8%, 58.4% and 49.7%, respectively, all higher than those of the control group, indicating that FeS2 could effectively reduce the dependence of the system on carbon sources. 3) The microbial community test results showed that FeS2 increased the species abundance of electrogenic microorganisms (Thauera, Thiobacillus and Geobacter) by about 9.43%. 4) The results of material transfer analysis showed that S− provided electrons for the denitrification process, and Fe2+ enhanced the electron transfer and improved the electro-generation performance of the system as an electron shuttle.
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Key words:
- microbial fuel cell /
- FeS2 /
- enhance /
- denitrification and nitrogen removal /
- electricity production
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图 1 MFC试验装置
1—磁力搅拌器；2—阳极室；3—阴极室；4—外电阻；5—阳离子交换膜；6—碳刷电极；7—数据采集系统；8—电脑。
Figure 1. MFC experimental setup
图 5 C/N对不同MFC脱氮效果的影响
Figure 5. Effect of C/N ratio on the denitrification effect of different MFCs
图 6 C/N对不同MFC产电效果影响
Figure 6. Effect of C/N ratio on the electricity production effect of different MFCs
图 8 微生物不同物种分类学水平的相对丰度
Figure 8. Relative abundance of microorganisms at the taxonomic level of different species
图 10 不同价态铁或硫在MFC中的微生物相对丰度
Figure 10. Relative microbial abundance of different valence states of iron or sulfur in MFC
表 1 微量元素溶液组成
Table 1. Composition of trace element solution
试剂名称 浓度/（g/L） CaCl2 1.00 MgCl2·6H2O 2.00 NaCl 0.20 FeCl2·4H2O 0.50 CoCl2·6H2O 0.10 MnCl2·4H2O 0.10 AlCl3·6H2O 0.05 (NH4)6Mo7O24·4H2O 0.30 H3BO3 0.10 NiCl2·6H2O 0.01 CuSO4·5H2O 0.10 ZnCl2 0.10 EDTA 0.50 
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