Research and application advances of Anammox in nitrogen removal from municipal wastewater
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摘要:
厌氧氨氧化（anaerobic ammonium oxidation，Anammox）是城市污水处理工艺未来最具发展潜力的新型脱氮技术之一。基于Anammox反应机理，归纳了在城市污水条件下实现短程硝化耦合Anammox (partial-nitritation coupled Anammox，PN/A)和短程反硝化耦合Anammox (partial-denitrification coupled Anammox，PD/A) 的运行调控措施，阐述了Anammox强化城市污水脱氮的工艺模式，并从工程化应用角度总结了阻碍城市污水实现Anammox处理的瓶颈问题，同时对PN/A与PD/A的脱氮性能、降耗减排效果、工艺特征及应用场景进行了对比分析，最后总结并展望了Anammox在城市污水处理中的研究方向，以期为城市污水处理工艺向Anammox转变提供借鉴和参考。
Abstract:Anaerobic ammonium oxidation (Anammox) is one of the most promising new nitrogen removal technologies for municipal wastewater in the future. Based on Anammox reaction mechanism, the operation control strategies of partial-nitrification coupled Anammox (PN/A) and partial-denitrification coupled Anammox (PD/A) under the condition of municipal wastewater were summarized, and the process modes of Anammox to strengthen the denitrification in municipal wastewater were elaborated. At the same time, the bottleneck problems hindering the realization of Anammox treatment from the perspective of engineering application were concluded. Finally, the nitrogen removal performance, effect of reducing consumption and emission, process characteristics and application scenarios of PN/A and PD/A in municipal wastewater were compared and analyzed, and the future research direction of Anammox was summarized and prospected, so as to provide reference for the transformation of urban sewage treatment to Anammox process.
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图 2 基于PN/A技术的氮去除路径
注：黑色实线为短程硝化（PN），紫色实线为Anammox。DBa为A类反硝化菌(以NO2 −为终产物)，DBb为B类反硝化菌(无NO2 −积累)，DBc为C类反硝化菌(NO2 −短暂积累)。
Figure 2. Nitrogen removal path based on PN/A technology
图 4 基于PN路线的城市污水侧流Anammox工艺模式
Figure 4. Sidestream Anammox process model of urban sewage based on PN route
图 5 基于PD/A技术的氮去除路径
注：黑色实线为硝化过程，蓝色实线为PD，紫色实线为Anammox。
Figure 5. Nitrogen removal path based on PD/A
图 6 基于PD路线的主流Anammox亚硝酸盐积累策略
Figure 6. Nitrite accumulation strategy based on PD route in mainstream Anammox
表 1 PN/A与PD/A的脱氮性能及降耗减排情况
Table 1. Performance of nitrogen removal, consumption and discharge reduction of PN/A and PD/A
指标 含义 PN/A PD/A 数据来源 NO2 −积累率/% >90 >90 文献[51-52] 理论氮去除率/% 对无机氮的去除率 89 100 文献[53] 碳源消耗量/(g/g) 每g NH4 +-N消耗的碳源（以COD计） 0.31 1.31 文献[12] 有机物回收量/kg 理论上从1.0×105 m3城市污水(NH4 +-N浓度=40 mg/L，COD=400 mg/L)中回收的COD量 22 964 22 517 文献[53] 耗氧量/(g/g) 每g NH4 +-N的耗氧量 1.95 2.39 文献[12] 污泥产量/(g/g) 每g NH4 +-N的可挥发性固体（VSS）产生量 0.14 0.29 文献[12] CO2排放量/(g/g) 每g NH4 +-N的CO2排放量 较少 0.79 文献[7,54] N2O排放量/% N2O排放量占去除氮负荷的比例 1.35±0.72 0.074~0.66 文献[55-56] 
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表 2 Anammox应用在城市污水处理厂的代表性工程案例
Table 2. Representative engineering cases of Anammox application in municipal wastewater treatment plants
污水处理厂名称 处理规模
/(m3/d)脱氮路线 核心工艺 Anammox
处理模式运行温度/℃ 国外 奥地利Strass污水处理厂[57] 2.6×104 PN/A DEMON 侧流 30~40 荷兰Dokhaven污水处理厂[24] 3.4×105 PN/A SHARON-
Anammox侧流 30~40 美国Blue Plains污水处理厂[58] 1.4×106 PN/A DEMON 侧流 30~40 瑞士Zürich污水处理厂[59] PN/A CANON 侧流 30~40 新加坡Changi再生污水处理厂[60] 2.0×105 PN/A 多级A/O-
分段进水主流 28~32 国内 北京高碑店污水处理厂[4] 1.0×106 PN/A IFAS 侧流 30~40 西安第四污水处理厂[61] 2.5×105 PD/A A/A/O-
MBBR主流 11~25
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[1] WANG Z B, LIU X L, BU C N, et al. Microbial diversity reveals the partial denitrification-anammox process serves as a new pathway in the first mainstream anammox plant[J]. Science of the Total Environment,2021,764:142917. doi: 10.1016/j.scitotenv.2020.142917 [2] 万莉, 邹义龙, 弓晓峰, 等.电增强零价铁强化厌氧氨氧化处理高氮养猪废水[J]. 环境科学研究,2015,28(8):1302-1310. doi: 10.13198/j.issn.1001-6929.2015.08.18WAN L, ZOU Y L, GONG X F, et al. Electrical field and zero-valent iron-enhanced anaerobic ammonium oxidation for the treatment of high-nitrogen swine wastewater[J]. Research of Environmental Sciences,2015,28(8):1302-1310. doi: 10.13198/j.issn.1001-6929.2015.08.18 [3] DAVEREY A, SU S H, HUANG Y T, et al. Partial nitrification and anammox process: a method for high strength optoelectronic industrial wastewater treatment[J]. Water Research,2013,47(9):2929-2937. doi: 10.1016/j.watres.2013.01.028 [4] HAN X Y, ZHANG S J, YANG S H, et al. Full-scale partial nitritation/anammox (PN/A) process for treating sludge dewatering liquor from anaerobic digestion after thermal hydrolysis[J]. Bioresource Technology,2020,297:122380. doi: 10.1016/j.biortech.2019.122380 [5] 王茜, 陈琴, 曾涛涛, 等.基于短程硝化工艺的垃圾渗滤液脱氮处理研究进展[J]. 环境工程技术学报,2016,6(2):127-132. doi: 10.3969/j.issn.1674-991X.2016.02.019WANG X, CHEN Q, ZENG T T, et al. Review of nitrogen removal for landfill leachate based on partial nitrification technology[J]. Journal of Environmental Engineering Technology,2016,6(2):127-132. doi: 10.3969/j.issn.1674-991X.2016.02.019 [6] JI J T, PENG Y Z, LI X Y, et al. A novel partial nitrification-synchronous anammox and endogenous partial denitrification (PN-SAEPD) process for advanced nitrogen removal from municipal wastewater at ambient temperatures[J]. Water Research,2020,175:115690. doi: 10.1016/j.watres.2020.115690 [7] DU R, CAO S B, ZHANG H Y, et al. Flexible nitrite supply alternative for mainstream anammox: advances in enhancing process stability[J]. Environmental Science & Technology,2020,54(10):6353-6364. [8] KARTAL B, de ALMEIDA N M, MAALCKE W J, et al. How to make a living from anaerobic ammonium oxidation[J]. FEMS Microbiology Reviews,2013,37(3):428-461. doi: 10.1111/1574-6976.12014 [9] STROUS M, HEIJNEN J J, KUENEN J G, et al. The sequencing batch reactor as a powerful tool for the study of slowly growing anaerobic ammonium-oxidizing microorganisms[J]. Applied Microbiology and Biotechnology,1998,50(5):589-596. doi: 10.1007/s002530051340 [10] JETTEN M S M, STROUS M, van de PAS-SCHOONEN K T, et al. The anaerobic oxidation of ammonium[J]. FEMS Microbiology Reviews,1998,22(5):421-437. doi: 10.1111/j.1574-6976.1998.tb00379.x [11] KUENEN J G. Anammox bacteria: from discovery to application[J]. Nature Reviews Microbiology,2008,6(4):320-326. doi: 10.1038/nrmicro1857 [12] ZHANG M, WANG S Y, JI B, et al. Towards mainstream deammonification of municipal wastewater: partial nitrification-anammox versus partial denitrification-anammox[J]. Science of the Total Environment,2019,692:393-401. doi: 10.1016/j.scitotenv.2019.07.293 [13] MIAO Y Y, ZHANG J H, PENG Y Z, et al. An improved start-up strategy for mainstream anammox process through inoculating ordinary nitrification sludge and a small amount of anammox sludge[J]. Journal of Hazardous Materials,2020,384:121325. doi: 10.1016/j.jhazmat.2019.121325 [14] LI X J, SUN S, YUAN H Y, et al. Mainstream upflow nitritation-anammox system with hybrid anaerobic pretreatment: long-term performance and microbial community dynamics[J]. Water Research,2017,125:298-308. doi: 10.1016/j.watres.2017.08.048 [15] RAHMAN A, MEERBURG F A, RAVADAGUNDHI S, et al. Bioflocculation management through high-rate contact-stabilization: a promising technology to recover organic carbon from low-strength wastewater[J]. Water Research,2016,104:485-496. doi: 10.1016/j.watres.2016.08.047 [16] CAGNETTA C, SAERENS B, MEERBURG F A, et al. High-rate activated sludge systems combined with dissolved air flotation enable effective organics removal and recovery[J]. Bioresource Technology,2019,291:121833. doi: 10.1016/j.biortech.2019.121833 [17] BUDYCH-GORZNA M, SZATKOWSKA B, JAROSZYNSKI L, et al. Towards an energy self-sufficient resource recovery facility by improving energy and economic balance of a municipal WWTP with chemically enhanced primary treatment[J]. Energies,2021,14(5):1445. doi: 10.3390/en14051445 [18] 马斌. 城市污水连续流短程硝化厌氧氨氧化脱氮工艺与技术[D]. 哈尔滨: 哈尔滨工业大学, 2012. [19] LIU G Q, WANG J M. Long-term low DO enriches and shifts nitrifier community in activated sludge[J]. Environmental Science & Technology,2013,47(10):5109-5117. [20] GILBERT E M, AGRAWAL S, BRUNNER F, et al. Response of different Nitrospira species to anoxic periods depends on operational DO[J]. Environmental Science & Technology,2014,48(5):2934-2941. [21] REGMI P, MILLER M W, HOLGATE B, et al. Control of aeration, aerobic SRT and COD input for mainstream nitritation/denitritation[J]. Water Research,2014,57:162-171. doi: 10.1016/j.watres.2014.03.035 [22] YANG Q, PENG Y Z, LIU X H, et al. Nitrogen removal via nitrite from municipal wastewater at low temperatures using real-time control to optimize nitrifying communities[J]. Environmental Science & Technology,2007,41(23):8159-8164. [23] CAO Y S, KWOK B H, YAN Z, 等.新加坡最大回用水处理厂污水短程硝化厌氧氨氧化脱氮工艺[J]. 北京工业大学学报,2015,41(10):1441-1454.CAO Y S, HONG K B, YAN Z, et al. Mainstream partial nitritation/anammox nitrogen removal process in the largest water reclamation plant in Singapore[J]. Journal of Beijing University of Technology,2015,41(10):1441-1454. [24] HELLINGA C, SCHELLEN A A J C, MULDER J W, et al. The Sharon process: an innovative method for nitrogen removal from ammonium-rich waste water[J]. Water Science and Technology,1998,37(9):135-142. doi: 10.2166/wst.1998.0350 [25] SOLER-JOFRA A, WANG R, KLEEREBEZEM R, et al. Stratification of nitrifier guilds in granular sludge in relation to nitritation[J]. Water Research,2019,148:479-491. doi: 10.1016/j.watres.2018.10.064 [26] BARTROLÍ A, PÉREZ J, CARRERA J. Applying ratio control in a continuous granular reactor to achieve full nitritation under stable operating conditions[J]. Environmental Science & Technology,2010,44(23):8930-8935. [27] BIAN W, ZHANG S Y, ZHANG Y Z, et al. Achieving nitritation in a continuous moving bed biofilm reactor at different temperatures through ratio control[J]. Bioresource Technology,2017,226:73-79. doi: 10.1016/j.biortech.2016.12.014 [28] POOT V, HOEKSTRA M, GELEIJNSE M A A, et al. Effects of the residual ammonium concentration on NOB repression during partial nitritation with granular sludge[J]. Water Research,2016,106:518-530. doi: 10.1016/j.watres.2016.10.028 [29] CORBALÁ-ROBLES L, PICIOREANU C, van LOOSDRECHT M C M, et al. Analysing the effects of the aeration pattern and residual ammonium concentration in a partial nitritation-Anammox process[J]. Environmental Technology,2016,37(6):694-702. doi: 10.1080/09593330.2015.1077895 [30] YAO Z B, CAI Q, ZHANG D J, et al. The enhancement of completely autotrophic nitrogen removal over nitrite (CANON) by N2H4 addition[J]. Bioresource Technology,2013,146:591-596. doi: 10.1016/j.biortech.2013.07.121 [31] XU G J, XU X C, YANG F L, et al. Partial nitrification adjusted by hydroxylamine in aerobic granules under high DO and ambient temperature and subsequent Anammox for low C/N wastewater treatment[J]. Chemical Engineering Journal,2012,213:338-345. doi: 10.1016/j.cej.2012.10.014 [32] WANG Y Y, WANG Y W, WEI Y S, et al. In-situ restoring nitrogen removal for the combined partial nitritation-anammox process deteriorated by nitrate build-up[J]. Biochemical Engineering Journal,2015,98:127-136. doi: 10.1016/j.bej.2015.02.028 [33] DU R, CAO S B, LI B K, et al. Step-feeding organic carbon enhances high-strength nitrate and ammonia removal via DEAMOX process[J]. Chemical Engineering Journal,2019,360:501-510. doi: 10.1016/j.cej.2018.12.011 [34] MA B, QIAN W T, YUAN C S, et al. Achieving mainstream nitrogen removal through coupling anammox with denitratation[J]. Environmental Science & Technology,2017,51(15):8405-8413. [35] 马斌, 许鑫鑫, 高茂鸿, 等.基于短程反硝化厌氧氨氧化的低碳源城市污水深度脱氮特性[J]. 环境科学,2020,41(3):1377-1383.MA B, XU X X, GAO M H, et al. Advanced nitrogen removal characteristics of low carbon source municipal wastewater treatment via partial-denitrification coupled with ANAMMOX[J]. Environmental Science,2020,41(3):1377-1383. [36] 殷同昕, 操家顺, 张腾, 等.不同碳源下反硝化亚硝酸盐积累情况研究进展[J]. 应用化工,2020,49(11):2919-2925. doi: 10.3969/j.issn.1671-3206.2020.11.053YIN T X, CAO J S, ZHANG T, et al. Research progress on nitrite accumulation during denitrification with different carbon sources[J]. Applied Chemical Industry,2020,49(11):2919-2925. doi: 10.3969/j.issn.1671-3206.2020.11.053 [37] GONG L X, HUO M X, YANG Q, et al. Performance of heterotrophic partial denitrification under feast-famine condition of electron donor: a case study using acetate as external carbon source[J]. Bioresource Technology,2013,133:263-269. doi: 10.1016/j.biortech.2012.12.108 [38] LE T, PENG B, SU C Y, et al. Impact of carbon source and COD/N on the concurrent operation of partial denitrification and anammox[J]. Water Environment Research,2019,91(3):185-197. doi: 10.1002/wer.1016 [39] 袁怡, 黄勇, 邓慧萍, 等.C/N比对反硝化过程中亚硝酸盐积累的影响分析[J]. 环境科学,2013,34(4):1416-1420.YUAN Y, HUANG Y, DENG H P, et al. Effect of C/N ratio on nitrite accumulation during denitrification process[J]. Environmental Science,2013,34(4):1416-1420. [40] CAO S B, DU R, LI B K, et al. Nitrite production from partial-denitrification process fed with low carbon/nitrogen (C/N) domestic wastewater: performance, kinetics and microbial community[J]. Chemical Engineering Journal,2017,326:1186-1196. doi: 10.1016/j.cej.2017.06.066 [41] DU R, PENG Y Z, JI J T, et al. Partial denitrification providing nitrite: opportunities of extending application for Anammox[J]. Environment International,2019,131:105001. doi: 10.1016/j.envint.2019.105001 [42] 李思倩, 路立, 王芬, 等.低温反硝化过程中pH对亚硝酸盐积累的影响[J]. 环境化学,2016,35(8):1657-1662. doi: 10.7524/j.issn.0254-6108.2016.08.2016011105LI S Q, LU L, WANG F, et al. Effect of pH on nitrite accumulation during denitrification at low temperature[J]. Environmental Chemistry,2016,35(8):1657-1662. doi: 10.7524/j.issn.0254-6108.2016.08.2016011105 [43] 操沈彬. 基于短程反硝化的厌氧氨氧化脱氮工艺与菌群特性[D]. 哈尔滨: 哈尔滨工业大学, 2018. [44] GLASS C, SILVERSTEIN J. Denitrification kinetics of high nitrate concentration water: pH effect on inhibition and nitrite accumulation[J]. Water Research,1998,32(3):831-839. doi: 10.1016/S0043-1354(97)00260-1 [45] QIAN W T, MA B, LI X Y, et al. Long-term effect of pH on denitrification: high pH benefits achieving partial-denitrification[J]. Bioresource Technology,2019,278:444-449. doi: 10.1016/j.biortech.2019.01.105 [46] LIU B B, MAO Y J, BERGAUST L, et al. Strains in the genus Thauera exhibit remarkably different denitrification regulatory phenotypes[J]. Environmental Microbiology,2013,15(10):2816-2828. [47] 厉巍. 高效短程反硝化耦合厌氧氨氧化工艺研究[D]. 杭州: 浙江大学, 2017. [48] 张星星, 王超超, 王垚, 等.基于不同废污泥源的短程反硝化快速启动及稳定性[J]. 环境科学,2020,41(8):3715-3724.ZHANG X X, WANG C C, WANG Y, et al. Rapid start-up and stability of partial denitrification based on different waste sludge sources[J]. Environmental Science,2020,41(8):3715-3724. [49] LI J W, PENG Y Z, ZHANG L, et al. Quantify the contribution of Anammox for enhanced nitrogen removal through metagenomic analysis and mass balance in an anoxic moving bed biofilm reactor[J]. Water Research,2019,160:178-187. doi: 10.1016/j.watres.2019.05.070 [50] DU R, CAO S B, LI B K, et al. Performance and microbial community analysis of a novel DEAMOX based on partial-denitrification and anammox treating ammonia and nitrate wastewaters[J]. Water Research,2017,108:46-56. doi: 10.1016/j.watres.2016.10.051 [51] DU R, CAO S B, LI B K, et al. Synergy of partial-denitrification and anammox in continuously fed upflow sludge blanket reactor for simultaneous nitrate and ammonia removal at room temperature[J]. Bioresource Technology,2019,274:386-394. doi: 10.1016/j.biortech.2018.11.101 [52] WANG H, XU G J, QIU Z, et al. NOB suppression in pilot-scale mainstream nitritation-denitritation system coupled with MBR for municipal wastewater treatment[J]. Chemosphere,2019,216:633-639. doi: 10.1016/j.chemosphere.2018.10.187 [53] CAO S B, OEHMEN A, ZHOU Y. Denitrifiers in mainstream anammox processes: competitors or supporters[J]. Environmental Science & Technology,2019,53(19):11063-11065. [54] 柴宏祥, 杨世琪, 何强, 等.污水生物处理脱氮工艺的温室气体排放比较[J]. 给水排水,2014,50(7):129-134. doi: 10.3969/j.issn.1002-8471.2014.07.033 [55] 廖正伟, 贺酰淑, 陈宣, 等.pH值对短程反硝化及N2O释放特性影响[J]. 西安建筑科技大学学报(自然科学版),2019,51(4):605-609.LIAO Z W, HE X S, CHEN X, et al. Effect of pH values on shortcut denitrification and nitrous oxide emission[J]. Journal of Xi'an University of Architecture & Technology (Natural Science Edition),2019,51(4):605-609. [56] ALI M, RATHNAYAKE R M L D, ZHANG L, et al. Source identification of nitrous oxide emission pathways from a single-stage nitritation-anammox granular reactor[J]. Water Research,2016,102:147-157. doi: 10.1016/j.watres.2016.06.034 [57] 郝晓地, 程慧芹, 胡沅胜.碳中和运行的国际先驱奥地利Strass污水厂案例剖析[J]. 中国给水排水,2014,30(22):1-5.HAO X D, CHENG H Q, HU Y S. International pioneer of carbon-neutral operation of wastewater treatment: a case study at Strass in Austria[J]. China Water & Wastewater,2014,30(22):1-5. [58] AL-OMARI A, HAN M F, WETT B, et al. Main-stream deammonification evaluation at blue Plains advanced wastewater treatment plant (AWTP)[J]. Proceedings of the Water Environment Federation,2012,2012(15):1959-1967. doi: 10.2175/193864712811725636 [59] JOSS A, DERLON N, CYPRIEN C, et al. Combined nitritation-anammox: advances in understanding process stability[J]. Environmental Science & Technology,2011,45(22):9735-9742. [60] CAO Y S, KWOK B H, van LOOSDRECHT M C M, et al. The occurrence of enhanced biological phosphorus removal in a 200 000 m3/day partial nitration and Anammox activated sludge process at the Changi water reclamation plant, Singapore[J]. Water Science and Technology,2017,75(3/4):741-751. [61] 苑泉, 贺北平, 钱亮, 等.某污水厂主流Anammox现象产生的原因探讨[J]. 中国给水排水,2020,36(11):1-8.YUAN Q, HE B P, QIAN L, et al. Discussion on reasons of mainstream Anammox phenomenon in a wastewater treatment plant[J]. China Water & Wastewater,2020,36(11):1-8. ⊗ -
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