石油烃降解菌的筛选及其降解特性研究

冒学宇; 李勇; 吴丛杨慧; 蔡凯; 林雪峰

doi:10.12153/j.issn.1674-991X.20210203

石油烃降解菌的筛选及其降解特性研究

doi: 10.12153/j.issn.1674-991X.20210203

冒学宇^{1, 2,},
李勇^1, ,,
吴丛杨慧²,
蔡凯²,
林雪峰^{1, 2}

1.
苏州科技大学环境科学与工程学院
2.
苏州市宏宇环境科技股份有限公司

基金项目: 国家水体污染控制与治理科技重大专项（2017ZX07205-002）

详细信息

作者简介:
冒学宇（1996—），女，硕士研究生，主要研究方向为水污染控制理论与技术研究，mxy15501691531@163.com

通讯作者:
李勇（1969—），男，教授，主要从事废水生物处理理论与技术研究，yongli69@163.com

中图分类号: X172，X53
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- 文章访问数: 411
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- 被引次数: 0
出版历程
- 收稿日期: 2021-05-26
- 网络出版日期: 2022-06-07

Research on the screening of petroleum hydrocarbon degrading bacteria and their degradation characteristics

MAO Xueyu^{1, 2
,},
LI Yong^{1
, ,},
WU Congyanghui²,
CAI Kai²,
LIN Xuefeng^{1, 2}

1.
School of Environmental Science and Engineering, Suzhou University of Science and Technology
2.
Suzhou Hongyu Environmental Technology Co., Ltd.

摘要

摘要:
从连云港某废弃化工厂污染土壤中分离筛选高效石油烃降解菌株，研究菌株的生理生化特征并对其进行测序和种属鉴定，采用单因素试验对菌株降解柴油的环境因子进行分析。结果表明：从污染土壤中共分离出柴油降解菌株4株，经过测序及同源比对，与该4株菌株同源性最高的分别为阴沟肠杆菌（Enterobacter_cloacae，HY1），肺无色杆菌（Achromobacter_pulmonis，HY2），台湾假单胞菌（Pseudomonas_taiwanensis，HY3），铜绿假单胞菌（Pseudomonas_aeruginosa，HY4），同源性均达98%以上；4株菌株具备不同的产表面活性剂能力和柴油降解能力，其中菌株HY1和HY2对柴油的降解率最高，当柴油浓度为0.5%，处理时间为20 d时，二者对柴油的降解率均达37%以上；通过单因素试验对降解条件进行优化后，发现在柴油浓度为0.5%，降解时间为8 d时，菌株HY2和HY1最佳降解条件是初始pH为7，摇床转速为180 r/min，接种量为3%~4%，此时，二者对柴油的降解率分别为40.15%和43.87%。本研究可以丰富石油烃降解菌的菌种信息，为石油烃污染土壤修复提供菌种资源及数据支持。
- 微生物降解 /
- 柴油降解菌 /
- 降解特性 /
- 筛选分离 /
- 菌株鉴定
Abstract:
The efficient petroleum hydrocarbon degrading bacteria were isolated and screened from the polluted soil of an abandoned chemical plant in Lianyungang City. The physiological and biochemical characteristics of the strains were studied, sequenced and identified. The related environmental factors of diesel fuel degradation were analyzed by single factor test. The results showed that four strains of diesel degrading bacteria were isolated from polluted soil. After sequencing and homology comparison, the highest homology with the four strains were Enterobacter_cloacae (HY1), Achromobacter_pulmonis (HY2), Pseudomonas_taiwanensis (HY3), Pseudomonas_aeruginosa (HY4), and the homology was more than 98%. The four strains had different abilities of producing surfactant and degrading diesel oil. HY1 and HY2 had the highest degradation rate of diesel oil. When the diesel oil concentration was 0.5% and the treatment time was 20 days, the degradation rate of diesel oil by the two strains was more than 37%. After optimizing the degradation conditions by single factor experiment, when the diesel oil concentration was 0.5% and the degradation time was 8 days, the optimal degradation conditions of HY2 and HY1 were initial pH 7, shaking table speed 180 r/min and inoculation amount 3%-4%. At this time, the degradation rates of diesel oil by the two strains were 40.15% and 43.87%, respectively. This study can enrich the strain information of petroleum hydrocarbon degrading bacteria and provide strain resources and data support for the remediation of petroleum hydrocarbon contaminated soil.
- microbial degradation /
- diesel oil degrading bacteria /
- degradation characteristics /
- screening and separation /
- strain identification

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图 1 4株菌的系统发育树

Figure 1. Phylogenetic tree of 4 strains

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图 2 产表面活性剂试验结果

Figure 2. Surfactant production results

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图 3 各菌株的柴油降解率

Figure 3. Diesel degradation rate of each strain

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图 4 菌株HY1、HY2和HY4在不同柴油浓度平板的生长状态

注：数字为柴油浓度。

Figure 4. Growth state of plate with different diesel oil concentration of strains HY1, HY2 and HY4

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图 5 菌株HY1、HY2、HY4的生长曲线

Figure 5. Growth curve of strains HY1, HY2, HY4

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图 6 菌株HY1、HY2在不同pH时对柴油的降解率

Figure 6. Degradation rate of diesel oil by strains HY1 and HY2 at different pH

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图 7 菌株HY1、HY2在不同转速时对柴油的降解率

Figure 7. Degradation rate of diesel oil by strains HY1 and HY2 at different speeds

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图 8 菌株HY1、HY2在不同接种量时对柴油的降解率

Figure 8. Degradation rate of diesel oil by strains HY1 and HY2 under different inoculation amounts

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表 1 4株菌株理化性质

Table 1. Physicochemical properties of 4 strains

鉴定项目	HY1	HY2	HY3	HY4
菌体形态	杆状	杆状	杆状	杆状
菌落形态	白色、黏液状	圆形、淡黄色、湿润	扁平、白色、湿润	湿润、微绿
革兰氏染色	−	−	−	−
吲哚试验	+	+	+	+
柠檬酸钠	+	++	−	++
甲基红	−	−	−	−
VP试验	++	++	++	++
明胶液化	+	+	+	+
接触酶试验	+	+	+	+
注：+表示阳性，++表示强阳性，−表示阴性。

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表 2 4株菌的序列比对信息

Table 2. Sequence alignment information of 4 strains

菌株	同源微生物名称	同源性/%
HY1	阴沟肠杆菌（Enterobacter cloacae）	98
HY2	肺无色杆菌（Achromobacter pulmonis）	99
HY3	台湾假单胞菌（Pseudomonas taiwanensis）	99
HY4	铜绿假单胞菌（Pseudomonas aeruginosa）	99

下载: 导出CSV

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