天津临港滨海湿地公园水体细菌种群特征

马香菊; 徐慧韬; 王丽平

doi:10.12153/j.issn.1674-991X.20200259

天津临港滨海湿地公园水体细菌种群特征

doi: 10.12153/j.issn.1674-991X.20200259

国家环境保护河口与海岸带环境重点实验室,中国环境科学研究院

详细信息

作者简介:
马香菊(1994—),女,硕士研究生,主要研究方向为海洋生态,mxiangju2020@163.com

通讯作者:
王丽平 E-mail: wanglp@craes.org.cn

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出版历程
- 收稿日期: 2020-11-03
- 刊出日期: 2021-05-20

Characteristics of bacterial population of water from Tianjin Lingang Coastal Wetland Park

State Environmental Protection Key Laboratory of Estuarine and Coastal Environment, Chinese Research Academy of Environmental Sciences

More Information

Corresponding author: WANG Liping E-mail: wanglp@craes.org.cn

摘要

摘要: 为了解天津临港湿地公园不同季节不同功能区水体细菌种群及其差异特征,采用高通量测序技术分析了湿地进水口、潜流湿地区、景观水体区及出水口水体中夏秋季的细菌种群结构,并采用主成分分析法分析其主要环境影响因素。结果表明:细菌Shannon多样性指数夏季为4.25~5.14,秋季为3.07~4.32,种群丰富度Chao指数夏季为569.10~894.04,秋季为473.22~995.81,整体上细菌多样性夏季高于秋季,其中夏季潜流湿地区细菌多样性和丰富度最高,秋季湿地进水口细菌多样性和丰富度最高;夏季共检测到细菌34门、423属、641种,主要优势门为变形菌门、放线菌门、拟杆菌门、蓝细菌门,主要优势属为红细菌属、噬氢菌属、norank_f_FamilyⅠ;秋季共检测到细菌34门、520属、786种,主要优势门为变形菌门、放线菌门、拟杆菌门,主要优势属为红细菌属、嗜冷杆菌属、聚球藻属;在优势属中,嗜氢菌属只在夏季潜流湿地区具有较高的相对丰度,嗜冷杆菌属只在秋季潜流湿地区具有较高的相对丰度,表明这些菌属为潜流湿地区特有菌种;基于OTU水平的物种Venn图发现,湿地公园不同区域夏季细菌共有种为187种,秋季为110种;基于OTU水平下物种组成与环境因子间的主成分分析结果表明,影响湿地公园细菌种群组成的主要环境因子为水温、pH与$NO_3^-$-N、NH₃-N浓度。
- 滨海湿地公园 /
- 高通量测序 /
- 细菌 /
- 种群特征
Abstract: To understand the bacterial community and its difference characteristics of water bodies in different functional areas of Tianjin Lingang Coastal Wetland Park in different seasons, the high-throughput sequencing technology was used to analyze the bacterial population structure of water from the intake, subsurface wetland, landscape water area and the water outlet of the wetland park in summer and autumn, and the principal component analysis was used to analyze its main environmental influential factors. The results showed that Shannon diversity index of bacteria ranged from 4.25 to 5.14 in summer and from 3.07 to 4.32 in autumn, Chao index of population abundance ranged from 569.10 to 894.04 in summer and from 473.22 to 995.81 in autumn. Overall, Shannon diversity index was higher in summer than that in autumn. And the highest bacterial diversity and richness in summer was in subsurface wetland, while in autumn it was in water intake. A total of 34 phyla, 423 genera, and 641 species of bacteria were detected in summer. The main dominant phyla included Proteobacteria, Actinobacteria, Bacteroidetes, Cyanobacteria, and the main dominant genera included Rhodobacter, Hydrogenophaga, norank_f_FamilyⅠ. In comparison, a total of 34 phyla, 520 genera, and 786 species of bacteria were detected in autumn. The main dominant phyla included Proteobacteria, Actinomycetes, Actinobacteria, Bacteroidetes, and the main dominant genera includedRhodobacter, Psychrobacter, Synechococcus. Among the dominant genera, the relative abundance of Hydrogenophaga was high only in the subsurface wetland in summer, and that of Psychrobacter was high only in the subsurface wetland in autumn. However, they were not detected in other area, indicating that these genera were unique in the subsurface wetland. The Venn map of species based on OTU levels showed that there were 187 common species in different areas of the wetland park in summer, and 110 species in autumn. The principal component analysis based on OTU level between species composition and environmental factors showed that the main environmental factors affecting the bacterial population composition of wetland parks included temperature, pH, $NO_3^-$-N, and NH₃-N.
- coastal wetland park /
- high-throughput sequencing /
- bacteria /
- population characteristics

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