巢湖沉积物中有机磷的生物可利用性研究

卢艳敏; 张靖天; 张新波; 肖喆; 许晓玲; 霍守亮

doi:10.12153/j.issn.1674-991X.20190125

巢湖沉积物中有机磷的生物可利用性研究

doi: 10.12153/j.issn.1674-991X.20190125

卢艳敏^1,2,
张靖天²,
张新波^1,*, ,,
肖喆²,
许晓玲²,
霍守亮²

^1. 天津城建大学环境与市政工程学院
^2. 中国环境科学研究院

详细信息

作者简介:
卢艳敏(1995—),女,硕士研究生,主要研究方向为湖泊富营养化控制,luyanmin2013@163.com

通讯作者:
张新波 E-mail: zxbcj2006@126.com

中图分类号: X524
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出版历程
- 收稿日期: 2019-07-15
- 刊出日期: 2020-03-20

Bioavailability of organic phosphorus in Lake Chaohu sediments

^1. School of Environmental and Municipal Engineering, Tianjin Chengjian University
^2. Chinese Research Academy of Environmental Sciences

More Information

Corresponding author: Xinbo ZHANG E-mail: zxbcj2006@126.com

摘要

摘要: 沉积物中有机磷通过转化为无机磷被藻类利用,加速水体富营养化进程。采集巢湖沉积物柱状样品,用Ivanoff连续提取法分级提取不同深度沉积物中有机磷,测定了沉积物中碱性磷酸酶的活性,并研究了碱性磷酸酶对沉积物中碳酸氢钠提取态有机磷(NaHCO₃-Po)、盐酸提取态有机磷(HCl-Po)、氢氧化钠提取态有机磷(NaOH-Po)的水解效果。结果表明:东半湖区C14采样点的有机磷浓度高于西半湖区C4采样点,C4采样点NaOH-Po、HCl-Po、NaHCO₃-Po和残渣态-Po浓度占总有机磷浓度的比例分别为41.70%、12.82%、11.05%和2.22%,C14采样点分别为43.75%、19.00%、6.12%和 4.22%,2个采样点不同形态有机磷浓度均为NaOH-Po>HCl-Po>NaHCO₃-Po>残渣态-Po;C4采样点的碱性磷酸酶活性高于C14采样点,这与西半湖区富营养化程度较高有关;NaHCO₃-Po、HCl-Po、NaOH-Po均能被碱性磷酸酶水解,C4和C14采样点表层沉积物中各形态有机磷的水解比例显著高于下层沉积物,说明人类活动产生的有机磷生物可利用性较高,其中,以简单小分子化合物为主的NaHCO₃-Po被水解的比例最高,为65.78%~69.47%。
- 生物可利用性 /
- 有机磷 /
- 碱性磷酸酶 /
- 水解
Abstract: Organic phosphorus in sediments can be converted into inorganic phosphorus, which may accelerate lake eutrophication process. Sectional sediment samples were collected in Lake Chaohu, organic phosphorus in sediments were extracted by Ivanoff method, and alkaline phosphatase activity in sediments were determined. The hydrolysis effects of alkaline phosphatase on NaHCO₃ extracted Po (NaHCO₃-Po), HCl extracted Po (HCl-Po) and NaOH extracted Po (NaOH-Po) in sediments were studied. The results indicated that Po content at C14 sampling station in the eastern half of the lake was higher than that at C4 sampling station in the western half of the lake. The proportion of NaOH-Po, HCl-Po, NaHCO₃-Po and Residual-Po in total organic phosphorus was 41.70%, 12.82%, 11.05% and 2.22%, respectively at C4 sampling station, 43.75%, 19.00%, 6.12% and 4.22%, respectively at C4 sampling station, and the concentrations of different forms of organic phosphorus were in the order of NaOH-Po>HCl-Po>NaHCO₃-Po>Residual-Po. The alkaline phosphatase activity at C4 sampling station was higher than that at C14 sampling station due to high eutrophication level in the western half of Lake Chaohu. NaHCO₃-Po, HCl-Po and NaOH-Po could be hydrolyzed by alkaline phosphatase, and the hydrolysis contents of various organic phosphorus in surface layers at C4 and C14 stations were significantly higher than those in deep layers, indicating that the bioavailability of organic phosphorus produced by human activities were higher. Indeed, the hydrolysis ratio of NaHCO₃-Po was highest with 65.78%-69.47% averaged values due to its simple and small molecular compounds.
- bioavailability /
- organic phosphorus /
- alkaline phosphatase /
- hydrolysis

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