三维荧光光谱表征Fe(Ⅱ)浓度对厌氧氨氧化过程的影响与平行因子分析

王嵘; 姚亮; 申慧彦; 余丽; 李卫华

doi:10.12153/j.issn.1674-991X.2019.09.120

三维荧光光谱表征Fe(Ⅱ)浓度对厌氧氨氧化过程的影响与平行因子分析

doi: 10.12153/j.issn.1674-991X.2019.09.120

王嵘^1,2,
姚亮^1,2,
申慧彦^1,2,
余丽^1,2,
李卫华^1,2, ,

^1. 安徽建筑大学环境与能源工程学院,安徽合肥 230022
^2. 水污染控制与废水资源化安徽省重点实验室,安徽合肥 230022

详细信息

作者简介:
王嵘(1989—),女,硕士,主要研究方向为废水生物处理, ronger@ahjzu.edu.cn

通讯作者:
李卫华 E-mail: liweihua9@126.com

中图分类号: X703
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出版历程
- 收稿日期: 2019-07-08
- 刊出日期: 2019-11-20

Characterizing the effect of Fe(Ⅱ) dosage on anammox treatment process using excitation-emission matrix fluorescence spectroscopy and parallel factor analysis

WANG Rong^1,2,
YAO Liang^1,2,
SHEN Huiyan^1,2,
YU Li^1,2,
LI Weihua^{1,2
, ,}

^1. School of Environmental and Energy Engineering, Anhui Jianzhu University, Hefei 230022, China
^2. Key Laboratory of Water Pollution Control and Wastewater Reuse of Anhui Province, Hefei 230022, China

More Information

Corresponding author: Weihua LI E-mail: liweihua9@126.com

摘要

摘要: 考察Fe(Ⅱ)浓度对厌氧氨氧化过程的影响,并采用三维荧光光谱结合平行因子分析方法,解析厌氧氨氧化反应器出水中的荧光组分,探究外加Fe(Ⅱ)与反应器出水水质的关系。结果表明:随着Fe(Ⅱ)浓度从1.84 mg/L升至5.00 mg/L,N $H 4 +$ -N和N $O 2 -$ -N的去除率逐渐增加,表明增加进水Fe(Ⅱ)浓度可以提高微生物对底物的利用率;随着Fe(Ⅱ)浓度的增加,厌氧氨氧化菌的数量亦显著增加;厌氧氨氧化反应器出水的主要荧光组分是类蛋白质和类富里酸物质,随着Fe(Ⅱ)浓度的增加,反应器出水的类蛋白质荧光强度显著增强,表明在一定条件下,投加Fe(Ⅱ)可以促进厌氧氨氧化菌的生长。因此,利用三维荧光光谱法可以反映投加Fe(Ⅱ)对厌氧氨氧化性能的影响,进而反映反应器实际运行状况。
- 厌氧氨氧化 /
- 荧光光谱 /
- 平行因子分析 /
- Fe(Ⅱ); /
- 类蛋白质
Abstract: The effect of ferrous ions dosage on the anaerobic ammonia was investigated. The excitation-emission matrix (EEM) fluorescence spectroscopy combined with parallel factor analysis (PARAFAC) method was used to decompose the EEM fluorescence components in the effluent samples. The relationship between the added Fe(Ⅱ) and the effluent quality of the reactor was explored. The results showed that with the increase of ferrous ions concentration from 1.84 mg/L to 5.00 mg/L, the removal rates of N $H 4 +$ -N and N $O 2 -$ -N increased gradually, indicating that increasing the influent ferrous ions concentration could increase the utilization rate of substrate by microorganisms. The proportion of anammox bacteria increased significantly with the increase of ferrous ions dosage; protein-like substances and fulvic acid-like substances were the main fluorophores in the effluent of anammox reactor. With the increase of ferrous ions dosage, the protein-like fluorescence of the effluents increased significantly, which implicated that the dosage of ferrous ions would promote the growth of anammox bacteria under certain conditions. Therefore, EEM fluorescence spectroscopy could reflect the effect of ferrous ions on the performance of anaerobic ammonia oxidation, which then implicate the actual operation of the reactor.
- anammox /
- fluorescence spectra /
- parallel factor analysis(PARAFAC) /
- Fe(Ⅱ); /
- protein-like substance

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