辽河保护区人工湿地微生物群落结构及分布规律

姚美辰; 段亮; 张恒亮; 张承铭

doi:10.12153/j.issn.1674-991X.2019.03.040

辽河保护区人工湿地微生物群落结构及分布规律

doi: 10.12153/j.issn.1674-991X.2019.03.040

中国环境科学研究院城市水环境研究室,北京 100012

详细信息

作者简介:
姚美辰(1995—),女,硕士研究生,研究方向为水环境处理技术, 626435458@qq.com

通讯作者:
段亮 E-mail: duanliang@craes.org.cn

中图分类号: X703
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出版历程
- 收稿日期: 2018-09-25
- 刊出日期: 2019-05-20

Microbial community structure and distribution of constructed wetlands in Liaohe Conservation Area

Department of Urban Water Environmental Research, Chinese Research Academy of Environmental Sciences, Beijing 100012, China

More Information

Corresponding author: Liang DUAN E-mail: duanliang@craes.org.cn

摘要

摘要: 利用高通量测序技术,研究了辽河保护区盘锦段3种类型人工湿地(支流汇入口湿地、坑塘湿地、牛轭湖湿地)底泥和芦苇根际微生物群落结构与分布规律。结果表明: 3种类型人工湿地底泥微生物多样性、丰度和操作分类单元(optical transform units,OTUs)数目均大于芦苇根际微生物,且底泥和芦苇根际微生物多样性受季节变化影响,秋季高于春季;不同类型人工湿地微生物群落结构及分布大致相似,变形菌门(Proteobacteria)为相对丰度最高的优势种,以变形菌门为主的革兰氏阴性菌在人工湿地净化过程中起重要作用;不同类型人工湿地变形菌纲及其相对丰度不同,支流汇入口湿地以α-变形菌纲(Alphaproteobacteria)、δ-变形菌纲(Deltaproteobacteria)和γ-变形菌纲(Gammaproteobacteria)为主;坑塘湿地以γ-变形菌纲和α-变形菌纲为主;牛轭湖湿地中不同纲的微生物分布较均匀。
- 辽河保护区 /
- 人工湿地 /
- 高通量测序 /
- 微生物群落 /
- 分布规律
Abstract: The high-throughput sequencing was used to study the microbial community structure and distribution of sediment and reed rhizosphere in different types of constructed wetlands (tributary inlet wetland, pond wetland and oxbow lake wetland) in Panjin of Liaohe Conservation Area. The results showed that the microbial diversity, abundance and optical transform units (OTUs) of the three kinds of wetlands sediment were higher than that of reed rhizosphere. The diversity was affected by season, and the diversity of samples in autumn was higher than that in spring. The structure and distribution of microbial communities were roughly similar. Proteobacteria was the dominant species with the highest relative abundance. Gram-negative bacteria, mainly proteobacteria, played an important role in the purification of constructed wetlands. The class and relative abundance of Proteobacteria in different types of constructed wetlands were different. The Alphaproteobacteria, Deltaproteobacteria and Gammaproteobacteria were dominated in the tributary inlet wetland, while the Gammaproteobacteria, and Alphaproteobacteria were dominated in the pond wetland. In the oxbow lake wetland, the microorganism distribution of different classes in wetland was uniform.
- Liaohe Conservation Area /
- constructed wetland /
- high-throughput sequencing /
- microbial community structure /
- distribution pattern

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