Abstract: Woody garden waste is rich in lignin and low in available nutrients, often facing difficulties such as low decomposition efficiency by microorganisms and the inability of the composting mass to achieve full maturation during composting. Using crushed wood chips from woody garden waste as the main raw material for composting and a mixture of microbial flora and molasses waste liquid as the composting regulator for composting, this study investigated the characteristics and ripening indexes of the compost. The research results showed that by adding the compost regulator in different composting stages, the composting temperature could effectively increase, triggering a secondary fermentation, and the high-temperature period lasted for up to 20 days. At the end of the composting process, the nutrients in the compost were as follows: the organic matter content was 666.3 mg/g, the total nitrogen content was 15.6 mg/g, and the available phosphorus content was 57.2 mg/kg. Of these nutrients, the total nitrogen content increased by 37.6% compared with the control group. The C/N ratio of the compost decreased from the initial 33 to 24.8 at the end of the composting process, and the seed germination index reached 88.2%, indicating that the compost had reached the maturity standard. However, for the control group without the addition of the compost regulator, the C/N ratio was 55.6 at the end of the composting process, and the germination index was only 32.5%, meaning that the composting was not completed. The RDA analysis showed that the addition of the regulator effectively changed the microbial community structure during the composting fermentation process, and Pseudomonadota bacteria became the dominant microbial species. In conclusion, the compost regulator composed of compost maturity microbial agents and molasses waste liquid can effectively initiate the composting of woody garden waste, accelerate the fermentation process, and promote the composting to reach maturity.