Abstract: In order to overcome the difficulties of direct resource utilization resulted from the C/N/P ratio imbalance, poor biochemical degradability and high concentration of TN and TP in anaerobic fermentation biogas residue, and to provide technical support for promoting green roof in the sponge city construction under low temperature environment in northern China, the proportion of sandy soil and biogas residue in the substrate was adjusted to cultivate Sedum lineare Thunb and the growth state observed. The experimental result indicated that the biogas residue reduced the bulk density, lightened the roof loads, increased the porosity of matrix and improved root penetration of Sedum lineare Thunb. In addition, the biogas residue promoted Sedum lineare Thunb growth under low temperature, increased height, coverage, tillering ability and chlorophyll content. The average quality assessment scores were all higher than 6, showing that Sedum lineare Thunb grew normally basically. The best comprehensive quality of Sedum lineare Thunb was reached when the biogas residue to sandy soil ratio for the substrate was 4:5 under medium and low temperatures.