Abstract: Taking Nansha New District of Guangzhou City as the study area, the framework of urban ecological protection red line division and management was established. Based on investigation and analysis of the ecological resource characteristics in the study area, the importance value and spatial distribution of the ecological service functions were evaluated. Based on the results of ecological service function evaluation, and combined with current situations of the land use, ecological protection and urban construction, the framework of ecological protection red lines was constructed. The study area was then divided into four levels, i.e. the red line area, the yellow line area, the blue line area and the green line area. According to ecological service function levels, their respective area scopes were divided, and the leading ecosystem services were recognized for each area. Furthermore, the ecological protection and environmental management requirements were proposed for all kinds of areas.