河床原位修复对城市河流微生物群落的影响

李赫龙; 黎双飞; 李伟奇; 林静

doi:10.3969/j.issn.1674-991X.2018.02.024

河床原位修复对城市河流微生物群落的影响

doi: 10.3969/j.issn.1674-991X.2018.02.024

^1. 深圳市碧园环保技术有限公司,广东深圳 518028
^2. 深圳大学生命与海洋科学学院,广东深圳 518060
^3. 深圳市百川检测有限公司,广东深圳 518000

详细信息

作者简介:
李赫龙(1990—),男,硕士,主要从事水环境与水生态修复研究,longlhbsd@163.com

中图分类号: X522
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- 文章访问数: 730
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- PDF下载量: 333
- 被引次数: 0
出版历程
- 收稿日期: 2017-10-30
- 刊出日期: 2018-03-20

Influence of riverbed in-situ remediation on microbial community in urban rivers

^1. Shenzhen Biyuan Environmental Protection Technology Co., Ltd., Shenzhen 518028, China
^2. College of Life and Ocean Sciences, Shenzhen University, Shenzhen 518060, China
^3. Shenzhen Branch Testing Co., Ltd., Shenzhen 518000, China

摘要

摘要: 采用分子生物学检测方法对实施了河床原位修复的河段进行全面的微生物群落分析。结果表明:河床生态修复后微生物群落结构及多样性发生了明显的变化。从微生物多样性指数来看,河床修复段的下游大于上游;从微生物群落结构来看,上游断面中ε-变形菌纲为最主要的细菌类型,多为致病病原菌,与该采样点周边人口密集而水质经其他处理有关。经河床原位修复后,下游断面ε-变形菌纲丰度减少,α-变形菌纲和β-变形菌纲成为最优势的类群,其他细菌比例也相对均衡,NH₃-N和其他理化指标有所好转,说明微生物载体原位修复措施对城市景观河流黑臭的改善有一定的效果。
- 景观河流 /
- 原位修复 /
- 微生物群落 /
- 16S rDNA
Abstract: An overall microbial community analysis was performed with molecular biological testing methods for the riverbed after in-situ remediation. The results showed that the microbial community structure and diversity were changed obviously after in-situ ecological remediation. In the microbial diversity index term, the lower reaches of the river after in-situ remediation were higher than the upstream reaches. In the microbial community structure term, ε-Proteobacteria were the most important bacteria in the upstream, and most of them were pathogenic bacteria, which were related to the surrounding dense population and untreated water. After in-situ remediation, ε-Proteobacteria abundance was reduced in the downstream, furthermore, α-Proteobacteria and β-Proteobacteria became the dominant groups in the lower reaches of the river, and the proportion of other bacteria was also relatively balanced. In addition,NH₃-N and other water quality indicators were improved. The research suggested that in-situ remediation technology has obvious effect on city landscape river ecological remediation.
- landscape river /
- in-situ remediation /
- microbial community /
- 16S rDNA

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参考文献(32)

[1]	苏彩燕, 周勤 . 珠江流域城市河道的综合整治建议与可持续发展[J].水利规划与设计, 2006(3):19-21.
[2]	李共国, 吴芝瑛, 虞左明 . 引水和疏浚工程对杭州西湖轮虫群落结构的影响[J]. 水生生物学报, 2007,31(3):386-392. LI G G, WU Z Y, YU Z M . Effects of drawing water and dredging on Rotifer Community in the West Lake,Hangzhou[J]. Acta Hydrobiologica Sinica, 2007,31(3):386-392.
[3]	王英才, 刘永定, 郝宗杰 , 等. 上海市几条黑臭河道治理效果的比较与分析[J]. 水生生物学报, 2009,33(2):355-359. WANG Y C, LIU Y D, HAO Z J , et al. Comparison and analysis the control effect of several black and stinking river in Shanghai[J]. Acta Hydrobiologica Sinica, 2009,33(2):355-359.
[4]	温新利, 席贻龙, 张雷 , 等. 芜湖市镜湖轮虫群落结构分析及水质的生态学评价[J]. 水生生物学报, 2006,30(2):152-158. WEN X L, XI Y L, ZHANG L , et al. Analysis of community structure of rotifer and ecological evaluation of water quality in lake Jinghu,Wuhu city[J]. Acta Hydrobiologica Sinica, 2006,30(2):152-158.
[5]	常素云 . 城市河道生物修复技术研究[D]. 天津:天津大学, 2011. CHANG S Y . Bio-remediation technology used on remediating the urban river[D]. Tianjin:Tianjin University, 2011.
[6]	吴小菁 . 城市河道底泥有机污染物生物化学联用修复技术研究[D]. 哈尔滨:哈尔滨工业大学, 2013. WU X J . Study on bio-chemical remediation technology for organic pollutants in urban river contaminated sediment[J]. Harbin:Harbin Institute of Technology, 2013.
[7]	李浩, 李鹏, 李波 , 等. MABR用于城市景观河道水体修复的研究[J]. 膜科学与技术, 2016,36(6):101-107. LI H, LI P, LI B , et al. Study on MABR application in urban river bioremediation water restoration of landscape[J]. Membrane Science and Technology, 2016,36(6):101-107.
[8]	刘晓伟, 谢丹平, 李开明 , 等. 城市河道底泥污染生物修复技术研究[J]. 生态科学, 2011,30(6):630-635. LIU X W, XIE D P, LI K M , et al. Study on bioremediation technology of polluted sediment in urban river[J]. Ecological Science, 2011,30(6):630-635.
[9]	王文林, 殷小海, 卫臻 , 等. 太阳能曝气技术治理城市重污染河道试验研究[J]. 中国给水排水, 2008,24(17):44-48. WANG W L, YIN X H, WEI Z , et al. Experimental study on solar aeration technology for treatment of heavily polluted urban river channel[J]. China Water & Wastewater, 2008,24(17):44-48.
[10]	王亚艳, 张剑刚, 倪鹏 , 等. 原位生态修复技术在城市河道中的应用[J]. 环境工程, 2015,33(3):11-16. WANG Y Y, ZHANG J G, NI P , et al. Application researches on in-situ ecological restoration technology in polluted urban river[J]. Environmental Engineering, 2015,33(3):11-16.
[11]	PELICERNACHER C, SMETS B F . Structure,composition,and strength of nitrifying membrane-aerated biofilms[J]. Water Research, 2014,57(12):151-161.
[12]	COLE A C, SEMMENS M J, LAPARA T M . Stratification of activity and bacterial community structure in biofilms grown on membranes transferring oxygen[J]. Applied & Environmental Microbiology, 2004,70(4):1982-1989.
[13]	姚丽平 . 城市黑臭河道底泥微生物群落结构对人工曝气的响应特征及机理研究[D]. 上海:华东师范大学, 2014. YAO L P . Study on mechanism and response of microbial community structure under different artificial aeration in urban black-odorous river sediment[D]. Shanghai:East China Normal University, 2014.
[14]	蔡林林, 周巧红, 王川 , 等. 南淝河细菌群落结构的研究[J]. 环境科学与技术, 2012,35(3):1-6. CAI L L, ZHOU Q H, WANG C , et al. Bacterial community structure of Nanfei River[J]. Environmental Science & Technology, 2012,35(3):1-6.
[15]	于鲁冀, 李廷梅, 刘攀龙 , 等. 微生物固定化技术在河流治理中的应用及研究进展[J]. 生物技术通报, 2016,32(8):56-61. YU L J, LI Y M, LIU P L , et al. Application of microbial immobilized technology in river pollution control and its research progress[J]. Biotechnology Bulletin, 2016,32(8):56-61.
[16]	邸攀攀, 张力, 王岩 , 等. 微生物固定化技术对污水中微生物丰度变化的影响[J]. 生态与农村环境学报, 2015,31(6):942-949. DI P P, ZHANG L, WANG Y , et al. Effect of microorganism immobilization techniques on microorganism abundances in polluted ponds[J]. Journal of Ecology and Rural Environment, 2015,31(6):942-949.
[17]	高岩, 张力, 王岩 , 等. 春季微生物固定化技术原位净化污染河塘的效果[J]. 江苏农业学报, 2015,31(2):334-341. GAO Y, ZHANG L, WANG Y , et al. Purification of polluted pond in situ by microorganism immobilization technology in spring[J]. Jiangsu Agricultural Sciences, 2015,31(2):334-341.
[18]	GOOD I L . The population frequencies of species and the estimation of population parameters[J]. Biometrika, 1953,40(3/4):237-264.
[19]	SUN J, LIU D Y . The application of diversity indices in marine phytoplankton studies[J]. Acta Oceanologica Sinica, 2004,26(1):62-75.
[20]	CHAO A . Estimating population size for sparse data in capture-recapture experiments[J]. Biometrics, 1989,45(2):427-438.
[21]	HOBAN D J, DOERN G V, FLUIT A C , et al. Worldwide prevalence of antimicrobial resistance in Streptococcus pneumoniae,Haemophilus influenzae,and Moraxella catarrhalis in the SENTRY Antimicrobial Surveillance Program,1997-1999[J]. Clinical Infectious Diseases:An Official Publication of the Infectious Diseases Society of America, 2001,32(2):81-93.
[22]	VAZMOREIRA I, NUNESO C, MANAIA C M . Diversity and antibiotic resistance patterns of sphingomonadaceae isolates from drinking water[J]. Applied & Environmental Microbiology, 2011,77(16):5697-5706.
[23]	ELIFANTZH, HORN G, AYON M , et al. Rhodobacteraceae are the key members of the microbial community of the initial biofilm formed in Eastern Mediterranean coastal seawater[J]. FEMS Microbiology Ecology, 2013,85(2):348-357.
[24]	范森, 段梦洁, 刘亚兰 , 等. 聚乳酸材料在不同土壤环境中生物降解的菌群结构分析[J]. 微生物学通报, 2017,44(10):2321-2329. FAN S, DUAN M J, LIU Y L , et al. Microbial diversity and community structure in biodegradation of poly(lactic) acid(PLA)in different soil environments[J]. Microbiology China, 2017,44(10):2321-2329.
[25]	杨茜 . 石油烃降解菌的筛选及石油污染土壤的生物修复机理研究[D]. 西安:西安建筑科技大学, 2014. YANG X . Screening of petroleum degrading strains and studying on the mechanisms of bioremediation of petroleum contaminated soil[D]. Xi’an:Xi’an University of Architecture & Technology, 2014.
[26]	郑李军, 傅明辉 . 水葫芦根际细菌群落结构多样性分析[J]. 微生物学通报, 2015,42(11):2115-2125. ZHENG L J, FU M H . Analysis of the diversity of bacterial community structure in the rhizosphere of Eichhornia crassipes[J]. Microbiology China, 2015,42(11):2115-2125.
[27]	有小娟 . 象山港养殖区生境修复的沉积环境细菌群落与环境影响因子的研究[D]. 青岛:中国海洋大学, 2013. YOU X J . Bacterial community and environmental influencing factors in rehabilitative sediment environment of Xiangshan Bay[D]. Qingdao:Ocean University of China, 2013.
[28]	黄廷林, 刘婷婷, 张海涵 , 等. 水库贫营养好氧反硝化细菌分离及其对水体微生物群落影响[J]. 西安建筑科技大学学报(自然科学版), 2012,44(6):876-882. HUANG T L, LIU T T, ZHANG H H , et al. Isolation of oligotrophic aerobic denitrifying bacteria and its effects on the water microbial communities[J]. Journal of Xi’an University of Architecture & Technology(Natural Science Edition), 2012,44(6):876-882.
[29]	PUJALTE M J, LUCENA T, RUVIR M A , et al. The family rhodobacteraceae[M]. Berlin Heidelberg:Springer, 2014: 439-512.
[30]	KALYUZHNAY M G, DE M P, BOWERMAN S , et al. Methyloversatilis universalis gen.nov.,sp. nov.,a novel taxon within the Betaproteobacteria represented by three methylotrophic isolates[J]. International Journal of Systematic and Evolutionary Microbiology, 2006,56(11):2517-2522.
[31]	KAMPFER P, BUSSE H J, ROSSELLO M R , et al. Rhodovarius lipocyclicus gen.nov.sp.nov.,a new genus of the α-1 subclass of the proteobacteria[J]. Systematic & Applied Microbiology, 2004,27(5):511-516.
[32]	ZHANG W, KI J S, QIAN P Y . Microbial diversity in polluted harbor sediments:Ⅰ.bacterial community assessment based on four clone libraries of 16S rDNA[J]. Estuarine Coastal & Shelf Science, 2008,76(3):668-681.