斑马鱼对3种氯酚的富集作用及其SOD酶活性应激反应研究

宋志慧; 孙欣欣; 李捍东

doi:10.3969/j.issn.1674-991X.2014.04.047

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斑马鱼对3种氯酚的富集作用及其SOD酶活性应激反应研究

doi: 10.3969/j.issn.1674-991X.2014.04.047

宋志慧^1,2,
孙欣欣²,
李捍东^1*, ,

1.
环境基准与风险评估国家重点实验室,中国环境科学研究院
2.
青岛科技大学环境与安全工程学院

详细信息

通讯作者:
李捍东 E-mail: li_han_dong@163.com

中图分类号: X174
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- 被引次数: 0
出版历程
- 收稿日期: 2014-01-24
- 修回日期: 2014-03-14
- 刊出日期: 2014-07-20

Study on Bioconcentration of Three Chlorophenols in Zebrafish and SOD Activity Stress Action

1.
State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences
2.
College of Environment and Safety Engineering, Qingdao University of Science & Technology

More Information

Corresponding author: LI Han-dong E-mail: li_han_dong@163.com

摘要

摘要: 为了研究对氯酚(4-CP)、2,4-二氯酚(2,4-DCP)、2,4,6-三氯酚(2,4,6-TCP)对斑马鱼的生理毒性作用，以7 d富集系数（K_BCF）和超氧化物歧化酶（SOD）活性为评价指标，采用半静态试验法对斑马鱼进行驯养和试验，测定斑马鱼对3种氯酚的7 d K_BCF，同时测定不同浓度条件下，暴露1 d和7 d，斑马鱼体内SOD酶活性。结果表明，4-CP、2,4-DCP和2,4,6-TCP的7 d K_BCF分别为33.65±5.29、65.54±10.49和128.05±39.83，富集能力表现为4-CP（无富集）＜2,4-DCP（轻度富集）＜2,4,6-TCP（轻度富集）；斑马鱼对氯代酚类物质的富集性与氯酚类物质氯原子取代个数正相关。3种氯酚胁迫斑马鱼结果表明，暴露1 d表现为诱导-抑制效应，暴露7 d表现为抑制效应，酶活性变化显著。
- 斑马鱼 /
- 对氯酚 /
- 2,4-二氯酚 /
- 2,4,6-三氯酚 /
- 超氧化物歧化酶
Abstract: To study the physio-toxicity effect of 4-chlorophenol, 2,4-dichlorophenol and 2,4,6-trichlorphenol on zebrafish, the evaluation indicator of 7 d K_BCF and SOD activity with different exposure concentrations were determined using zebrafish by semi-static test. The results showed that the 7 d K_BCF of 4-CP, 2,4-DCP and 2,4,6-TCP to zebrafish were 33.65±5.29,65.54±10.49 and 128.05±39.83, respectively, and the bioconcentration factor followed the order of 4-CP＜2,4-DCP＜2,4,6-TCP. The bioconcentration of the chlorophenols to zebrafish and the numbers of chloro derivatives had positive correlation. The results of stress action test of the chlorophenols to zebrafish showed that the effect at 1 d was induction-inhibition and at 7 d the effect was inhibition, indicating significant variation of SOD activity.
- zebrafish /
- 4-chlorophenol /
- 2,4-dichlorophenol /
- 2,4,6-trichlorphenol /
- SOD