Volume 13 Issue 2
Mar.  2023
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Article Navigation >  Journal of Environmental Engineering Technology  > 2023  >  13(2): 669-678
WEN H P,YANG X M,CHENG Y N,et al.Performance of a pilot-scale sequential batch reactor (SBR) on nitrogen and carbon removals and its characteristics of microbial community succession from biogas slurry from duck farm[J].Journal of Environmental Engineering Technology，2023，13（2）：669-678 doi: 10.12153/j.issn.1674-991X.20220057
Citation: WEN H P,YANG X M,CHENG Y N,et al.Performance of a pilot-scale sequential batch reactor (SBR) on nitrogen and carbon removals and its characteristics of microbial community succession from biogas slurry from duck farm[J].Journal of Environmental Engineering Technology，2023，13（2）：669-678 doi: 10.12153/j.issn.1674-991X.20220057
shu

Performance of a pilot-scale sequential batch reactor (SBR) on nitrogen and carbon removals and its characteristics of microbial community succession from biogas slurry from duck farm

doi: 10.12153/j.issn.1674-991X.20220057
  • 1.

    College of Natural Resources and Environment, Joint Institute for Environmental Research & Education, South ChinaAgricultural University

  • 2.

    Wens Foodstuff Group Co., Ltd.

  • 3.

    Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs

  • Received Date: 2022-01-20
    Abstract

  • A pilot-scale sequential batch reactor (SBR) was built in the duck farm, the duck farm biogas slurry was diluted as the influent, and the influent COD was regulated with sucrose, to evaluate the efficiency of nitrogen and carbon removal and the microbial community succession in the process of SBR treating the biogas slurry of the duck farm. The results showed that Stage Ⅰ (1-20 d) was the sludge inoculation and water quality adaptation stage, in which the influent C/N was controlled to be less than 2, and the concentrations of COD and NH4 +-N were around 200 mg/L. It was found that the removal efficiencies of COD and NH4 +-N rapidly increased to 80% and 90%, respectively, within the first 8th day. Stage Ⅱ (21-55 d) was the stabilization stage, in which C/N of the influent was also less than 2, and the concentrations of COD and NH4 +-N were 200-500 and 200-400 mg/L, respectively. In this stage, the removal efficiencies of COD were fluctuated around 60%, while the removal efficiencies of NH4 +-N were more than 80%. Stage Ⅲ (56-95 d) was the simulation stage for organic concentration change, in which C/N of the influent was in the range of 1.2 to 5.5, while the influent COD and NH4 +-N concentrations were 300-1 400 and 150-400 mg/L, respectively, and the removal rate of COD and NH4 +-N was greater than 80% in this stage. Meanwhile, low temperature was one of the main limiting factors for SBR nitrogen and carbon removal. Full-length sequencing of 16S rRNA from microorganisms revealed that Proteobacteria and Gammaproteobacteria were the dominant microbial flora at the phylum and class levels in the system, respectively. A significant shift of microbial community in terms of genus level was detected. The dominant microbial community species with nitrogen and carbon removal functions were formed after the operation stabilization. In general, SBR could achieve efficient nitrogen and carbon removal and had promising application potential in the real practice of treating biogas slurry from duck farm with a characteristics of high COD concentration and low C/N.

     

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