Volume 9 Issue 3
May  2019
Turn off MathJax
Article Contents
YAO Meichen, DUAN Liang, ZHANG Hengliang, ZHANG Chengming. Microbial community structure and distribution of constructed wetlands in Liaohe Conservation Area[J]. Journal of Environmental Engineering Technology, 2019, 9(3): 233-238. doi: 10.12153/j.issn.1674-991X.2019.03.040
Citation: YAO Meichen, DUAN Liang, ZHANG Hengliang, ZHANG Chengming. Microbial community structure and distribution of constructed wetlands in Liaohe Conservation Area[J]. Journal of Environmental Engineering Technology, 2019, 9(3): 233-238. doi: 10.12153/j.issn.1674-991X.2019.03.040

Microbial community structure and distribution of constructed wetlands in Liaohe Conservation Area

doi: 10.12153/j.issn.1674-991X.2019.03.040
More Information
  • Corresponding author: Liang DUAN E-mail: duanliang@craes.org.cn
  • Received Date: 2018-09-25
  • Publish Date: 2019-05-20
  • The high-throughput sequencing was used to study the microbial community structure and distribution of sediment and reed rhizosphere in different types of constructed wetlands (tributary inlet wetland, pond wetland and oxbow lake wetland) in Panjin of Liaohe Conservation Area. The results showed that the microbial diversity, abundance and optical transform units (OTUs) of the three kinds of wetlands sediment were higher than that of reed rhizosphere. The diversity was affected by season, and the diversity of samples in autumn was higher than that in spring. The structure and distribution of microbial communities were roughly similar. Proteobacteria was the dominant species with the highest relative abundance. Gram-negative bacteria, mainly proteobacteria, played an important role in the purification of constructed wetlands. The class and relative abundance of Proteobacteria in different types of constructed wetlands were different. The Alphaproteobacteria, Deltaproteobacteria and Gammaproteobacteria were dominated in the tributary inlet wetland, while the Gammaproteobacteria, and Alphaproteobacteria were dominated in the pond wetland. In the oxbow lake wetland, the microorganism distribution of different classes in wetland was uniform.

     

  • loading
  • [1]
    ZHAO Y J, LIU B, ZHANG W G , et al. Effects of plant and influent C:N:P ratio on microbial diversity in pilot-scale constructed wetlands[J]. Ecological Engineering, 2010,36(4):441-449.
    doi: 10.1016/j.ecoleng.2009.11.011
    [2]
    YUN J L, MA A Z, LI Y M , et al. Diversity of methanotrophs in Zoige wetland soils under both anaerobic and aerobic conditions[J]. Journal of Environmental Sciences, 2010,22(8):1232-1238.
    doi: 10.1016/S1001-0742(09)60243-6 pmid: 21179963
    [3]
    QIAN Y C, SHI J Y, CHEN Y X , et al. Characterization of phosphate solubilizing bacteria in sediments from a shallow eutrophic lake and a wetland: isolation, molecular identification and phosphorus release ability determination[J]. Molecules, 2010,15(11):8518-8533.
    doi: 10.3390/molecules15118518 pmid: 21102378
    [4]
    BAATH E . Effects of heavy metals in soil on microbial processes and populations:a review[J]. Water Air & Soil Pollution, 1989,47(3/4):335-379.
    [5]
    DONNELLY A P, HERBERT R A . Bacterial interactions in the rhizosphere of seagrass communities in shallow coastal lagoons[J]. Journal of Applied Microbiology, 1998,85(Suppl 1):151-160.
    doi: 10.1111/j.1365-2672.1998.tb05294.x pmid: 21182704
    [6]
    ZHI E Q, SONG Y H, DUAN L , et al. Spatial distribution and diversity of microbial community in large-scale constructed wetland of the Liao River Conservation Area[J]. Environmental Earth Sciences, 2015,73(9):5085-5094.
    doi: 10.1007/s12665-015-4021-7
    [7]
    RILEY D, BARBER S A . Bicarbonate accumulation and pH changes at the soybean (Glycine max(L.) Merr.) root-soil interface1[J]. Soil Science Society of America Journal, 1969,33(6):905-908.
    doi: 10.2136/sssaj1969.03615995003300060031x
    [8]
    史艳财, 邹蓉, 唐健民 , 等. 异裂菊属根际微生物群落结构及功能多样性[J]. 广西植物, 2018,38(1):1-10.

    SHI Y C, ZOU R, TANG J M , et al. Structural and functional diversity of rhizosphere microbial community of Heteroplex[J]. Guihaia, 2018,38(1):1-10.
    [9]
    杨瑞 . 挺水植物根际微生物生态研究[D]. 天津:天津大学, 2012.
    [10]
    金映虹, 吴红萍, 谭方正 , 等. 不同红树植物类群土壤微生物功能多样性的研究[J]. 生态环境学报, 2017,26(8):1292-1300.
    doi: 10.16258/j.cnki.1674-5906.2017.08.003

    JIN Y H, WU H P, TAN F Z , et al. Study on functional diversity of soil microorganism in different mangrove plants based on biolog-ECO technology[J]. Ecology & Environmental Sciences, 2017,26(8):1292-1300. doi: 10.16258/j.cnki.1674-5906.2017.08.003
    [11]
    赵萌, 印春生, 厉成伟 , 等. Miseq测序分析围垦后海三棱藨草湿地土壤微生物群落多样性的季节变化[J].上海海洋大学学报, 2018(5):718-727.

    ZHAO M, YIN C S, LI C W , et al. Using Miseq sequencing to analyze seasonal soil microbial community dynamics on reclaimed Scirpus mariqueter coastal wetlands[J].Journal of Shanghai Ocean University, 2018(5):718-727.
    [12]
    张骏达, 李素艳, 孙向阳 , 等. 基于高通量测序技术的不同年代公园绿地土壤细菌多样性[J]. 微生物学通报, 2019,46(1):65-74.

    ZHANG J D, LI S Y, SUN X Y , et al. Analysis of soil bacterial diversity in urban parks with different ages by high throughput sequencing[J]. Microbiology China, 2019,46(1):65-74.
    [13]
    李赫龙, 黎双飞, 李伟奇 , 等. 河床原位修复对城市河流微生物群落的影响[J]. 环境工程技术学报, 2018,8(2):176-184.

    LI H L, LI S F, LI W Q , et al. Influence of riverbed in-situ remediation on microbial community in urban rivers[J]. Journal of Environmental Engineering Technology, 2018,8(2):176-184.
    [14]
    谢冰, 董亮 . 芦苇人工湿地底泥微生物群落结构的PCR-SSCP技术研究[J].农业系统科学与综合研究, 2007(2):205-211.
    doi: 10.3969/j.issn.1001-0068.2007.02.019

    XIE B, DONG L . Study on microbial community structure of reed constructed wetland using PCR-SSCP[J].System Sciences & Comprehensive Studies in Agriculture, 2007(2):205-211. doi: 10.3969/j.issn.1001-0068.2007.02.019
    [15]
    车建锋, 李志斐, 王广军 , 等. 混养池塘生物膜微生物群落功能多样性特征分析[J]. 上海海洋大学学报, 2017,26(6):862-871.
    doi: 10.12024/jsou.20170302001

    CHE J F, LI Z F, WANG G J , et al. Microbial metabolic characteristics of biofilm communities in polyculture pond[J]. Journal of Shanghai Ocean University, 2017,26(6):862-871. doi: 10.12024/jsou.20170302001
    [16]
    ANSOLA G, ARROYO P, SÁENZ L E .Characterization of the soil bacterial community structure and composition of natural and constructed wetlands[J]. Science of the Total Environment, 2014,473(3):63-71.
    doi: 10.1016/j.scitotenv.2013.11.125 pmid: 24361449
    [17]
    邵颖, 曹四平, 刘长海 , 等. 基于高通量测序的南泥湾湿地土壤细菌多样性分析[J]. 干旱区资源与环境, 2019,33(2):158-163.

    SHAO Y, CAO S P, LIU C H , et al. Bacterial diversity in soils of Nanniwan wetland based on high-throughput sequencing[J]. Journal of Arid Land Resources and Environment, 2019,33(2):158-163.
    [18]
    宣丽霞, 戴文芳, 郁维娜 , 等. 有机污染物对杭州湾海域浮游细菌群落的影响[J]. 环境科学, 2018,39(8):3640-3648.
    doi: 10.13227/j.hjkx.201712186

    XUAN L X, DAI W F, YU W N , et al. Effects of organic pollutants on the bacterioplankton community in Hangzhou Bay[J]. Environmental Science, 2018,39(8):3640-3648. doi: 10.13227/j.hjkx.201712186
    [19]
    白洁, 李海艳, 赵阳国 . 黄海北部不同站位海洋细菌群落分布特征[J]. 微生物学报, 2009,49(3):343-350.
    doi: 10.3321/j.issn:0001-6209.2009.03.010

    BAI J, LI H Y, ZHAO Y G . Bacterial distribution at different stations in the Northern Yellow Sea[J]. Acta Microbiologica Sinica, 2009,49(3):343-350. doi: 10.3321/j.issn:0001-6209.2009.03.010
    [20]
    MIURA Y, WATANABE Y, OKABE S . Significance of Chloroflexi in performance of submerged membrane bioreactors (MBR) treating municipal wastewater[J]. Environmental Science & Technology, 2007,41(22):7787-7794.
    doi: 10.1021/es071263x pmid: 18075089
    [21]
    DUCKLOW H W, HILL S M . The growth of heterotrophic bacteria in the surface waters of warm core rings[J]. Limnology & Oceanography, 1985,30(2):239-259.
    doi: 10.4319/lo.1985.30.2.0239
    [22]
    TORTELL P D, MALDONADO M T, PRICE N M . The role of heterotrophic bacteria in iron-limited ocean ecosystems[J]. Nature, 1996,383(6598):330-332.
    doi: 10.1038/383330a0
    [23]
    王林, 李冰, 余家辉 , 等. 不同湿地模型中根系微生物的多样性[J]. 环境科学, 2017,38(8):3312-3318.
    doi: 10.13227/j.hjkx.201701030

    WANG L, LI B, YU J H , et al. Rhizosphere microbial diversity in different wetland microcosms[J]. Environmental Science, 2017,38(8):3312-3318. doi: 10.13227/j.hjkx.201701030
  • 加载中

Catalog

    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Article Metrics

    Article Views(389) PDF Downloads(140) Cited by()
    Proportional views
    Related

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return