Bacterial Diversity of the Combined Ozonation-ASBR/SBR Sludge Reduction with Simultaneous Nitrogen and Phosphorus
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摘要: 采用分子生物学手段PCR-DGGE技术对臭氧耦合ASBR/SBR控氮磷污泥减量化工艺中的ASBR和污泥减量化前后的SBR中的细菌多样性进行了研究。结果表明,ASBR中的主要细菌类群为Firmicutes类群,β- proteobacterium类群,γ-proteobacterium类群和Bacteroidetes类群;污泥减量化后SBR中细菌丰富度与减量前相比有所增加,但是相似性指数极高,为85%;臭氧施加进行污泥减量对SBR中的优势菌群多样性影响不大,污泥减量化前后SBR反应器内均存在Firmicutes bacterium,Candidate division TM7,ammonia-oxidizing bacterium,Bacteroidetes bacterium,β-proteobacterium,Denitrifying bacterium,Pseudomonas sp和Nitrosococcus halophilus Nc4,其中减量化后Bacteroidetes bacterium,β-proteobacterium,Denitrifying bacterium和Nitrosococcus halophilus Nc4的数量略高于污泥减量化之前。Abstract: The bacterial diversity in the ASBR and SBR of the combined process, i.e. combined ozonation-ASBR/SBR sludge reduction with simultaneous nitrogen and phosphorus removal process was studied by molecular biological PCR-DGGE method before and after sludge reduction. The results indicated that the dominant bacterial communities in the ASBR were Firmicutes bacterium, β-proteobacterium, γ-proteobacterium and Bacteroidetes bacterium. The bacterial richness in SBR after sludge reduction was slightly increased compared with that before sludge reduction, but with high similarity (85%). The dominant bacteria diversity of SBR was not influenced significantly by the ozone dosage for sludge reduction. There were Firmicutes bacterium, Candidate division TM7, ammonia-oxidizing bacterium, Bacteroidetes bacterium, β-proteobacterium, Denitrifying bacterium, Pseudomonas sp and Nitrosococcus halophilus Nc4 in SBR both before and after sludge reduction. However, the number of Bacteroidetes bacterium, β-proteobacterium, Denitrifying bacterium and Nitrosococcus halophilus Nc4 in SBR after sludge reduction was slightly higher than that before sludge reduction.
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Key words:
- ASBR/SBR /
- combined ozonation /
- sludge reduction /
- PCR-DGGE
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