Anoxic biodegradation of polycyclic aromatic hydrocarbons (PAHs) in aged deep soil pretreated with chemical oxidation
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摘要: 土壤中多环芳烃(PAHs)的化学氧化与微生物联合降解备受关注,但已有的研究主要集中在化学氧化与好氧微生物联用方面,与缺氧微生物联用研究较少。通过对过氧化氢氧化后的土壤进行接种和缺氧培养,考察化学氧化后深层土壤中PAHs缺氧微生物降解的可行性。结果表明:过氧化氢氧化后,16种PAHs降解了33.3%~95.9%,但土壤中细菌数量也明显减少,细菌数量的基因拷贝数减少了3.5个数量级;缺氧培养180 d后,添加营养盐可使微生物数量明显恢复,在同时添加营养盐和电子受体(硫酸盐)时微生物数量恢复程度更高;单独添加营养盐或电子受体不能明显促进PAHs降解,同时添加营养盐和电子受体能够促进PAHs降解,且同时添加营养盐和电子受体时,接种土壤中3环和部分4环PAHs(荧蒽和芘)的降解率显著高于未接种土壤。总体上,缺氧微生物降解可使PAHs去除率在化学氧化的基础上提高15%左右。Abstract: Integrated chemical-biological treatment of polycyclic aromatic hydrocarbons (PAHs) contaminated soil has received much attention. However, previous studies mainly focused on the remediation of soils contaminated with PAHs using oxidation treatment coupled with aerobic biodegradation, rather than anoxic biodegradation. In order to study the feasibility of applying combined chemical oxidation and anoxic biodegradation method to remediate PAHs contaminated soil, the anoxic culture of the soil oxidized by hydrogen peroxide was carried out. It was found that the removal efficiencies of 16 PAHs ranged from 33.3% to 95.9% after hydrogen peroxide oxidation, but the application of hydrogen peroxide resulted in a significant decrease of the number of bacteria in the soil, and the gene copy number of total bacteria in soil decreased by 3.5 orders of magnitude. However, bacterial numbers could be significantly recovered after 180 days of anoxic incubation in the treatment added with nutrients, and higher levels of bacteria were observed in treatments with nutrients and electron acceptor (sulfate) in inoculated soil. The use of nutrients or electron receptor (sulfate) alone could not enhance the biodegradation of PAHs obviously, but the application of both nutrients and electron acceptor could result in obviously biodegradation of PAHs. Moreover, higher biodegradation efficiency was observed for 3-rings and partial 4-rings PAHs (fluoranthene and pyrene) in inoculated soil than that of non-inoculated soil in treatment where both nutrients and electron acceptor were added. Overall, a further PAHs removal of about 15% was observed after anoxic biological treatment compared with chemical oxidation treatment alone.
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Key words:
- deep soil /
- PAHs /
- chemical oxidation /
- anoxic biodegradation /
- combined remediation
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[1] 侯晓鹏, 李春华, 叶春 , 等. 不同电子受体作用下微生物降解多环芳烃研究进展[J]. 环境工程技术学报, 2016,6(1):78-84.HOU X P, LI C H, YE C , et al. Research progress of biodegradation of polycyclic aromatic hydrocarbons with amendment of different electron acceptors[J]. Journal of Environmental Engineering Technology, 2016,6(1):78-84. [2] JONSSON S, PERSSON Y, FRANKKI S , et al. Degradation of polycyclic aromatic hydrocarbons (PAHs) in contaminated soils by Fenton’s reagent:a multivariate evaluation of the importance of soil characteristics and PAH properties[J]. Journal of Hazardous Materials, 2007,149(1):86-96. [3] SUTTON N B, GROTENHUIS J T C, LANGENHOFF A A M ,et al.Efforts to improve coupled in situ chemical oxidation with bioremediation:a review of optimization strategies[J]. Journal of Soils and Sediments, 2011,11(1):129-140. [4] KULIK N, GOI A, TRAPIDO M , et al. Degradation of polycyclic aromatic hydrocarbons by combined chemical pre-oxidation and bioremediation in creosote contaminated soil[J]. Journal of Environmental Management, 2006,78(4):382-391. [5] USMAN M, HANNA K, HADERLEIN S .Fenton oxidation to remediate PAHs in contaminated soils:a critical review of major limitations and counter-strategies[J].Science of the Total Environment, 2016, 569/ 570:179-190. [6] 张秋子, 韦云霄, 姜永海 , 等. 催化过氧化氢对石油烃污染土壤的氧化能力[J]. 环境工程技术学报, 2017,7(1):65-70.ZHANG Q Z, WEI Y X, JIANG Y H , et al. Oxidizing capacity of catalyzed hydrogen peroxide to petroleum hydrocarbon contaminated soil[J]. Journal of Environmental Engineering Technology, 2017,7(1):65-70. [7] CASSIDY D P, SRIVASTAVA V J, DOMBROWSKI F J , et al. Combining in situ chemical oxidation,stabilization,and anaerobic bioremediation in a single application to reduce contaminant mass and leachability in soil[J]. Journal of Hazardous Materials, 2015,297:347-355. [8] JHO E H, RYU H, SHIN D , et al. Prediction of landfarming period using degradation kinetics of petroleum hydrocarbons:test with artificially contaminated and field-aged soils and commercially available bacterial cultures[J]. Journal of Soils and Sediments, 2014,14(1):138-145. [9] CHEN K F, CHANG Y C, CHIOU W T . Remediation of diesel-contaminated soil using in situ chemical oxidation (ISCO) and the effects of common oxidants on the indigenous microbial community:a comparison study[J]. Journal of Chemical Technology and Biotechnology, 2016,91(6):1877-1888. [10] XU J L, DENG X, CUI Y W , et al. Impact of chemical oxidation on indigenous bacteria and mobilization of nutrients and subsequent bioremediation of crude oil-contaminated soil[J]. Journal of Hazardous Materials, 2016,320:160-168. [11] LU M, ZHANG Z Z, QIAO W , et al. Remediation of petroleum-contaminated soil after composting by sequential treatment with Fenton-like oxidation and biodegradation[J]. Bioresource Technology, 2010,101(7):2106-2113. [12] GHATTAS A K, FISCHER F, WICK A , et al. Anaerobic biodegradation of (emerging) organic contaminants in the aquatic environment[J]. Water Research, 2017,116:268-295. [13] ROCKNE K J, STRAND S E . Biodegradation of bicyclic and polycyclic aromatic hydrocarbons in anaerobic enrichments[J]. Environmental Science and Technology, 1998,32(24):3962-3967. [14] KRONENBERG M, TRABLY E, BERNET N , et al. Biodegradation of polycyclic aromatic hydrocarbons:using microbial bioelectrochemical systems to overcome an impasse[J]. Environmental Pollution, 2017,231(1):509-523. [15] GOU Y, YANG S, CHENG Y , et al. Enhanced anoxic biodegradation of polycyclic aromatic hydrocarbons (PAHs) in aged soil pretreated by hydrogen peroxide[J]. Chemical Engineering Journal, 2019,356:524-533. [16] CHANG B V, CHANG I T, YUAN S Y . Anaerobic degradation of phenanthrene and pyrene in mangrove sediment[J]. Bulletin of Environmental Contamination and Toxicology, 2008,80(2):145-149. [17] YANG S C, GOU Y L, SONG Y , et al. Enhanced anoxic biodegradation of polycyclic aromatic hydrocarbons (PAHs) in a highly contaminated aged soil using nitrate and soil microbes[J]. Environmental Earth Sciences, 2018,77(12):1-10. [18] 鲍士旦 . 土壤农化分析[M]. 北京: 中国农业出版社, 1999. [19] SUTTON N B, GROTENHUIS T, RIJNAARTS H H M .Impact of organic carbon and nutrients mobilized during chemical oxidation on subsequent bioremediation of a diesel-contaminated soil[J]. Chemosphere, 2014,97:64-70. [20] YAP C L, GAN S, NG H K . Fenton based remediation of polycyclic aromatic hydrocarbons-contaminated soils[J]. Chemosphere, 2011,83(11):1414-1430. [21] WATTS R J, STANTON P C, HOWSAWKENG J , et al. Mineralization of a sorbed polycyclic aromatic hydrocarbon in two soils using catalyzed hydrogen peroxide[J]. Science of the Total Environment, 2002,36(17):4283-4292. [22] SIRGUEY C, SILVA P T D, SCHWARTZ C ,et al.Impact of chemical oxidation on soil quality[J]. Chemosphere, 2008,72(2):282-289. [23] GONG X B . Remediation of weathered petroleum oil-contaminated soil using a combination of biostimulation and modified Fenton oxidation[J]. International Biodeterioration and Biodegradation, 2012,70(5):89-95. [24] JUNG H, AHN Y, CHOI H , et al. Effects of in-situ ozonation on indigenous microorganisms in diesel contaminated soil:survival and regrowth[J]. Chemosphere, 2005,61(7):923-32. [25] RICHARDSON S D, LEBRON B L, MILLER C T , et al. Recovery of phenanthrene-degrading bacteria after simulated in situ persulfate oxidation in contaminated soil[J]. Environmental Science and Technology, 2011,45(2):719-725. [26] XU J L, KONG F X, SONG S H , et al. Effect of Fenton pre-oxidation on mobilization of nutrients and efficient subsequent bioremediation of crude oil-contaminated soil[J]. Chemosphere, 2017,180(1):1-10. [27] LU X Y, ZHANG T, FANG H H . Bacteria-mediated PAH degradation in soil and sediment[J]. Applied and Environmental Microbiology, 2011,89(5):1357-1371. [28] PALMROTH M R T, LANGWALDT J H, AUNOLA T A , et al. Treatment of PAH-contaminated soil by combination of Fenton’s reaction and biodegradation[J]. Journal of Chemical Technology and Biotechnology, 2006,81(4):598-607. [29] VARJANI S J, UPASANI V N . A new look on factors affecting microbial degradation of petroleum hydrocarbon pollutants[J]. International Biodeterioration and Biodegradation, 2017,120:71-83. [30] CHIBWE L, GEIER M C, NAKAMURA J , et al. Aerobic bioremediation of PAH contaminated soil results in increased genotoxicity and developmental toxicity[J]. Environmental Science and Technology, 2015,49(23):13889-13898. [31] 孙明明, 滕应, 骆永明 . 厌氧微生物降解多环芳烃研究进展[J]. 微生物学报, 2012,52(8):931-939.SUN M M, TENG Y, LUO Y M . Progresses in anaerobic biodegradation of polycyclic aromatic hydrocarbons:a review[J]. Acta Microbiologica Sinica, 2012,52(8):931-939. [32] HOUSNA M, JORG J, MECKENSTOCK R U . Identification of naphthalene carboxylase as a prototype for the anaerobic activation of non-substituted aromatic hydrocarbons[J]. Environmental Microbiology, 2012,14(10):2770-2774. [33] DAVIDOVA I A, GIEG L M, DUNCAN K E , et al. Anaerobic phenanthrene mineralization by a carboxylating sulfate-reducing bacterial enrichment[J]. Isme Journal, 2007,1(5):436-442.
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