底物浓度对反硝化MBBR处理反渗透浓水脱氮效能及脱氮基因的影响

李莉; 闫国凯; 王海燕; 凌宇; 赵远哲; 王欢

doi:10.12153/j.issn.1674-991X.2019.04.260

底物浓度对反硝化MBBR处理反渗透浓水脱氮效能及脱氮基因的影响

doi: 10.12153/j.issn.1674-991X.2019.04.260

李莉¹,
闫国凯¹,
王海燕^1,2,*, ,,
凌宇¹,
赵远哲¹,
王欢¹

^1. 中国环境科学研究院环境污染控制技术工程研究中心,北京 100012
^2. 环境基准与风险评估国家重点实验室,中国环境科学研究院,北京 100012

详细信息

作者简介:
李莉(1994—),女,硕士研究生,研究方向为水污染控制工程,lili02@craes.org.cn

通讯作者:
王海燕 E-mail: wanghy@craes.org.cn

中图分类号: X703
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出版历程
- 收稿日期: 2019-04-20
- 刊出日期: 2019-07-20

Influence of substrate concentration on the nitrogen removal and relative genes of denitrifying MBBR for the treatment of reverse osmosis concentrate

LI Li¹,
YAN Guokai¹,
WANG Haiyan^{1,2,*
, ,},
LING Yu¹,
ZHAO Yuanzhe¹,
WANG Huan¹

^1. Research Center for Environmental Pollution Control Technology, Chinese Research Academy of Environmental Sciences,Beijing 100012, China
^2. State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing 100012, China

More Information

Corresponding author: Haiyan WANG E-mail: wanghy@craes.org.cn

摘要

摘要: 针对污水处理厂生产高品质再生水过程中低压反渗透单元(DFRO)产生的反渗透浓水中TN浓度高和N $O x -$ -N(N $O 3 -$ -N+N $O 2 -$ -N)占比高的问题,采用反硝化MBBR处理实际反渗透浓水,研究不同底物浓度下反硝化MBBR的脱氮效能和反硝化基因拷贝数的变化。结果表明:进水N $O 3 -$ -N浓度为(8.70±6.34)~(24.23±8.69)mg/L,TN浓度为(28.43±5.69)~(44.10±7.37)mg/L时,随着浓度的升高N $O 3 -$ -N和TN去除率保持平稳,但N $O 3 -$ -N和TN去除速率上升,N $O 2 -$ -N去除率和去除速率下降。进水N $O 2 -$ -N浓度为(10.94±8.51)~(20.94±5.78)mg/L时,随着浓度的升高,N $O 3 -$ -N和TN去除率及去除速率降低,N $O 2 -$ -N去除率及去除速率上升。反硝化MBBR填料生物膜主要由球菌、杆菌和少量丝状菌组成;填料生物膜和底泥中各脱氮基因拷贝数随N $O 3 -$ -N和TN浓度增加而增大,nirK、nirS和Anammox等基因拷贝数也随N $O 2 -$ -N浓度增加而增大。
- 反渗透浓水 /
- 反硝化MBBR /
- 底物浓度 /
- 脱氮基因 /
- N $O 2 -$ -N积累
Abstract: Denitrifying MBBR was used for the treatment of reverse osmosis concentrate with high TN and N $O x -$ -N (N $O 3 -$ -N and N $O 2 -$ -N) concentrations, which was generated from Dalton Filtration Reverse Osmosis (DFRO) unit in the high-quality water reclamation process of wastewater treatment plant effluent. The variation of denitrifying MBBR efficiency and the copy number of nitrogen removal genes were studied extensively under four different substrate concentrations. The results show that when the influent N $O 3 -$ -N and TN increased within the range of (8.70±6.34)-(24.23±8.69) and (28.43±5.69)-(44.10±7.37) mg/L, respectively, the N $O 3 -$ -N and TN removal ratios remained stable with the increase of removal rates, but the N $O 3 -$ -N and TN removal rates increased, while the N $O 2 -$ -N removal ratios and rates decreased. The N $O 3 -$ -N and TN removal ratios and rates decreased when the influent N $O 2 -$ -N concentration increased within the range of (10.94±8.51)-(20.94±5.78) mg/L, while the N $O 2 -$ -N removal ratios and rates increased. The biofilm on denitrifying MBBR carriers mainly consisted of cocci, bacillus and filamentous bacteria. The copy numbers of nitrogen removal genes in carrier biofilm and suspended sludge increased with the increase of influent N $O 3 -$ -N and TN concentration, and the copy numbers of nirK, nirS and Anammox genes also increased with the increase of influent N $O 2 -$ -N concentration.
- reverse osmosis concentrate /
- denitrifying MBBR /
- substrate concentration /
- nitrogen removal genes /
- N $O 2 -$ -N accumulation

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参考文献(38)

[1]	ΦDEGAARD H, MENDE U, SKJERPING E O , et al. Compact tertiary treatment based on the combination of MBBR and contained hollow fibre UF-membranes[J]. Desalination and Water Treatment, 2012,42(1/2/3):80-86.
[2]	RUSTEN B, HEM L J, ΦDEGAARD H . Nitrogen removal from dilute wastewater in cold climate using moving-bed biofilm reactors[J]. Water Environment Research, 1995,67(1):65-74. doi: 10.2175/106143095X131204
[3]	LABELLE M A, JUTEAU P, JOLICOEUR M , et al. Seawater denitrification in a closed mesocosm by a submerged moving bed biofilm reactor[J]. Water Research, 2005,39(14):3409-3417. doi: 10.1016/j.watres.2005.06.001
[4]	ΦDEGAARD H . Innovations in wastewater treatment:the moving bed biofilm process[J]. Water Science Technology, 2006,53(9):17-33.
[5]	杨岚, 彭永臻, 李健伟 , 等. 缺氧MBBR耦合部分厌氧氨氧化强化城市生活污水深度脱氮[J/OL]. 环境科学, 2019[ 2019-04-11]. https://doi.org/10.13227/j.hjkx. 201901015. YANG L, PENG Y Z, LI J W , et al. Advanced denitrification from municipal wastewater achieved via partial ANAMMOX in anoxic MBBR[J/OL]. Environmental Science, 2019[ 2019-04-11]. https://doi.org/10.13227/j.hjkx. 201901015.
[6]	苑泉, 王海燕, 刘凯 , 等. 污水厂尾水MBBR反硝化深度脱氮填料比较[J]. 环境科学学报, 2015,35(3):713-721. YUAN Q, WANG H Y, LIU K , et al. Comparison of the MBBR denitrification carriers for advanced nitrogen removal of wastewater treatment plant effluent[J]. Acta Scientiae Circumstantiae, 2015,35(3):713-721.
[7]	顾夏生, 胡洪营, 文湘华 , 等. 水处理生物学[M]. 北京: 中国建筑工业出版社, 2010: 244-246.
[8]	杨盈盈 . 进水渗滤液总氮和BOD₅/TN对填埋场反应器反硝化和厌氧氨氧化协同脱氮的影响[J]. 环境科学, 2015,36(4):1412-1416. YANG Y Y . Effects of total nitrogen and BOD5/TN on anaerobic ammonium oxidation-denitrification synergistic interaction of mature landfill leachate in aged refuse bioreactor[J]. Environmental Science, 2015,36(4):1412-1416.
[9]	康鹏亮, 黄廷林, 张海涵 , 等. 西安市典型景观水体水质及反硝化细菌种群结构[J]. 环境科学, 2017,38(12):5174-5183. KANG P L, HUANG T L, ZHANG H H , et al. Water quality and diversity of denitrifier community ctructure of typical scenic water bodies in Xi'an[J]. Environmental Science, 2017,38(12):5174-5183.
[10]	SHANNON M A, BOHN P W, ELIMELECH M , et al. Science and technology for water purification in the coming decades[J]. Nature, 2008,452(20):301-310.
[11]	程子芮, 姜云超, 王海燕 , 等. 二级MBBR处理城市污水高品质水再生过程产生的反渗透浓水填料最佳填充率研究[J]. 环境工程技术学报, 2017,7(3):285-292. CHENG Z R, JIANG Y C, WANG H Y , et al. Study on carrier optimum filling ratio of two-stage MBBR for treatment of reverse osmosis concentrate from high-quality water reclamation of WWTP effluent[J]. Journal of Environmental Engineering Technology, 2017,7(3):285-292.
[12]	吴迪 . MBBR在国内的工程应用与发展前景[J]. 中国给水排水, 2018,34(16):22-31. WU D . Application and development prospect of MBBR in China[J]. China Water & Wastewater, 2018,34(16):22-31.
[13]	李春梅, 王海燕, 王有乐 , 等. 液相化学处理MBBR填料对城市污水厂尾水深度脱氮的影响[J]. 环境工程技术学报, 2016,6(4):336-342. doi: 10.3969/j.issn.1674-991X.2016.04.050 LI C M, WANG H Y, WANG Y L , et al. Influence of the liquid-phase chemical method modified MBBR carriers on advanced nitrogen removal of urban wastewater treatment plant effluent[J]. Journal of Environmental Engineering Technology, 2016,6(4):336-342. doi: 10.3969/j.issn.1674-991X.2016.04.050
[14]	国家环境保护总局. 水和废水监测分析方法[M].4版. 中国环境科学出版社, 2002.
[15]	谢家仪, 董光军, 刘振英 . 扫描电镜的微生物样品制备方法[J]. 电子显微学报, 2005,24(4):440.
[16]	NOAH F, JASON A J, RYTAS V , et al. Assessment of soil microbial community structure by use of taxon-specific quantitative PCR assays[J]. Applied and Environmental Microbiology, 2005,71(7):4117-4120. doi: 10.1128/AEM.71.7.4117-4120.2005
[17]	WOUTER R L V D S, WIEBE R A, DENNIS B , et al. Startup of reactors for anoxic ammonium oxidation:experiences from the first full-scale anammox reactor in Rotterdam[J]. Water Research, 2007,41(18):4149-4163. doi: 10.1016/j.watres.2007.03.044
[18]	KIM Y M, LEE D S, PARK C , et al. Effects of free cyanide on microbial communities and biological carbon and nitrogen removal performance in the industrial activated sludge process[J]. Water Research, 2011,45(3):1267-1279. doi: 10.1016/j.watres.2010.10.003
[19]	SATOSHI I, NAOAKI A, SHIGETO O , et al. Isolation of oligotrophic denitrifiers carrying previously uncharacterized functional gene sequences[J]. Applied Environment Microbiology, 2011,77(1):338. doi: 10.1128/AEM.02189-10
[20]	STRES B, MUROVEC B . New primer combinations with comparable melting temperatures detecting highest numbers of nosZ sequences from sequence databases[J]. Acta Agriculturae Slovenica, 2009,94(2):139-142.
[21]	HANG Q Y, WANG H Y, CHU Z S , et al. Nitrate-rich agricultural runoff treatment by Vallisneria-sulfur based mixotrophic denitrification process[J]. Science of the Total Environment, 2017, 587/588:108-117.
[22]	王晖 . 硫自养反硝化结合生物活性炭技术处理硝酸氮污染水的研究[D]. 上海:上海交通大学, 2011.
[23]	王少坡, 彭永臻, 王淑莹 , 等. 不同硝态氮组成下反硝化过程控制参数pH变化规律[J]. 高技术通讯, 2005,15(8):91-95. WANG S P, PENG Y Z, WANG S Y , et al. Effect of proportion of nitrate and nitrite on pH profiles during denitrification[J]. High Technology Letters, 2005,15(8):91-95.
[24]	赵樑, 倪伟敏, 贾秀英 , 等. 初始pH值对废水反硝化脱氮的影响[J]. 杭州师范大学学报(自然科学版), 2014,13(6):616-622.
[25]	王亚宜, 王淑莹, 彭永臻 . MLSS、pH及N$O^{-}_{2}$-N对反硝化除磷的影响 [J]. 中国给水排水, 2005,21(7):47-51. WANG Y Y, WANG S Y, PENG Y Z . Influence of MLSS,pH,and N$O^{-}_{2}$-N concentration on denitrifying phosphorus removal [J]. China Water & Wastewater, 2005,21(7):47-51.
[26]	厉巍, 郑平, 谢作甫 , 等. 反硝化过程特性探析[J]. 科技通报, 2013,29(11):205-210. LI W, ZHENG P, XIE Z F , et al. Characteristics of denitrification process[J]. Bulletin of Science and Technology, 2013,29(11):205-210.
[27]	LI W, SHAN X Y, WANG Z Y , et al. Effect of self-alkalization on nitrite accumulation in a high-rate denitrification system:performance,microflora and enzymatic activities[J]. Water Research, 2016,88:758-765. doi: 10.1016/j.watres.2015.11.003
[28]	周明俊, 毛天广, 吴博 , 等. 厌氧氨氧化与反硝化耦合启动影响因素[J]. 供水技术, 2018,12(4):43-48. ZHOU M J, MAO T G, WU B , et al. Factors influencing the coupling start-up of ANAMMOX and denitrification[J]. Water Technology, 2018,12(4):43-48.
[29]	李亚峰, 王欣, 高颖 . 有机物、亚硝酸盐和pH值对反硝化脱氮除磷的影响[J]. 沈阳建筑大学学报(自然科学版), 2013,29(3):531-537. LI Y F, WANG X, GAO Y . Study on effects of different organic matter,nitrite and pH for denitrification nitrogen and phosphorus removal[J]. Journal of Shenyang Jianzhu University(Natural Science), 2013,29(3):531-537.
[30]	王亚宜, 彭永臻, 王淑莹 , 等. 碳源和硝态氮浓度对反硝化聚磷的影响及ORP的变化规律[J]. 环境科学, 2004,25(4):54-58. WANG Y Y, PENG Y Z, WANG S Y , et al. Effect of carbon source and nitrate concentration on denitrifying dephosphorus removal and variation of ORP[J]. Environmental Science, 2004,25(4):54-58.
[31]	方晶晶, 马传明, 刘存富 . 反硝化细菌研究进展[J]. 环境科学与技术, 2010,33(增刊1):206-210. FANG J J, MA C M, LIU C F . The advance of study on denitrifying Bacteria[J]. Environmental Science & Technology, 2010,33(Suppl 1):206-210.
[32]	CHEN Y, WEN Y, ZHOU Q , et al. Effects of plant biomass on denitrifying genes in subsurface-flow constructed wetlands[J]. Bioresource Technology, 2014,157(2):341-345. doi: 10.1016/j.biortech.2014.01.137
[33]	郑平, 张蕾 . 厌氧氨氧化菌的特性与分类[J]. 浙江大学学报(农业与生命科学版), 2009,35(5):473-481. ZHENG P, ZHANG L . Characterization and classification of anaerobic ammonium oxidation(anammox)bacteria[J]. Jounarl of Zhejiang University(Agriculture & Life Science), 2009,35(5):473-481.
[34]	BRAKER G, ZHOU J Z, WU L Y , et al. Nitrite reductase genes(nirK and nirS)as functional markers to investigate diversity of denitrifying bacteria in Pacific northwest marine sediment communities[J]. Applied Environmental Microbiology, 2000,66(5):2096-2104. doi: 10.1128/AEM.66.5.2096-2104.2000
[35]	MIAO Y, LIAO R, ZHANG X X , et al. Metagenomic insights into salinity effect on diversity and abundance of denitrifying bacteria and genes in an expanded granular sludge bed reactor treating high-nitrate wastewater[J]. Chemical Engineering Journal, 2015,277:116-123. doi: 10.1016/j.cej.2015.04.125
[36]	TORRESI E, GÜLAY A, POLESEL F , et al. Reactor staging influences microbial community composition and diversity of denitrifying MBBRs-implications on pharmaceutical removal[J]. Water Research, 2018,138:333-345. doi: 10.1016/j.watres.2018.03.014
[37]	WEI Z, JI G . Quantitative response relationships between nitrogen transformation rates and nitrogen functional genes in a tidal flow constructed wetland under C/N ratio constraints[J]. Water Research, 2014,64(7):32-41. doi: 10.1016/j.watres.2014.06.035
[38]	辛明秀, 赵颖, 周军 , 等. 反硝化细菌在污水脱氮中的作用[J]. 微生物学通报, 2007,34(4):773-776. XIN M X, ZHAO Y, ZHOU J , et al. The application of denitrifying bacteria in denitrification of wastewater[J]. Microbiology China, 2007,34(4):773-776.